Cellular cardiomyoplasty in a transgenic mouse model

Wilhelm Roell; Yun Fan; Ying Xia; Eva Stoecker; Philipp Sasse; Eugen Kolossov; Wilhelm Bloch; Harald Metzner; Christoph Schmitz; Klaus Addicks; Juergen Hescheler; Armin Welz; Bernd K Fleischmann

Cellular cardiomyoplasty in a transgenic mouse model

Wilhelm Roell, Yun Fan, Ying Xia, Eva Stoecker, Philipp Sasse, Eugen Kolossov, Wilhelm Bloch, Harald Metzner, Christoph Schmitz, Klaus Addicks, Juergen Hescheler, Armin Welz, Bernd K Fleischmann

Abteilung Molekulare und zelluläre Sportmedizin

Department of Cardiac Surgery, Medical Faculty, University of Bonn, Sigmund Freud Str. 25, 53105 Bonn, Germany. Electronic address: [email protected].

Publikation: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung

Abstract

BACKGROUND: Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality.

METHODS: Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter.

RESULTS: The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals.

CONCLUSIONS: This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.

Originalsprache	Englisch
Zeitschrift	Transplantation
Jahrgang	73
Ausgabenummer	3
Seiten (von - bis)	462-465
Seitenumfang	4
ISSN	0041-1337
Publikationsstatus	Veröffentlicht - 15.02.2002

Zitation

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title = "Cellular cardiomyoplasty in a transgenic mouse model",

abstract = "BACKGROUND: Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality.METHODS: Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter.RESULTS: The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals.CONCLUSIONS: This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.",

keywords = "Animals, Cardiomyoplasty, Green Fluorescent Proteins, Luminescent Proteins, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic",

author = "Wilhelm Roell and Yun Fan and Ying Xia and Eva Stoecker and Philipp Sasse and Eugen Kolossov and Wilhelm Bloch and Harald Metzner and Christoph Schmitz and Klaus Addicks and Juergen Hescheler and Armin Welz and Fleischmann, {Bernd K}",

year = "2002",

month = feb,

day = "15",

language = "English",

volume = "73",

pages = "462--465",

journal = "Transplantation",

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TY - JOUR

T1 - Cellular cardiomyoplasty in a transgenic mouse model

AU - Roell, Wilhelm

AU - Fan, Yun

AU - Xia, Ying

AU - Stoecker, Eva

AU - Sasse, Philipp

AU - Kolossov, Eugen

AU - Bloch, Wilhelm

AU - Metzner, Harald

AU - Schmitz, Christoph

AU - Addicks, Klaus

AU - Hescheler, Juergen

AU - Welz, Armin

AU - Fleischmann, Bernd K

PY - 2002/2/15

Y1 - 2002/2/15

N2 - BACKGROUND: Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality.METHODS: Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter.RESULTS: The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals.CONCLUSIONS: This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.

AB - BACKGROUND: Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality.METHODS: Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter.RESULTS: The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals.CONCLUSIONS: This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.

KW - Animals

KW - Cardiomyoplasty

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

M3 - Journal articles

C2 - 11884945

SN - 0041-1337

VL - 73

SP - 462

EP - 465

JO - Transplantation

JF - Transplantation

IS - 3

ER -

Cellular cardiomyoplasty in a transgenic mouse model

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