Changes of epigenetic modifications in natural killer cells of breast cancer patients in follow-up care by enhanced physical activity

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitrag - Abstract in KonferenzbandForschung


Natural killer cells (NK-cells) are able to recognize and kill tumor cells.
With different germ-line encoded receptors, they recognize tumor cells
and kill them by promoting cell lysation and apoptosis. A higher expression
of receptors on the surface of NK-cells could imply a higher sensitivity
to target cells.
Besides genetically settings, gene expression is also based on epigenetic
modifications. Whereas DNA methylation seems to have a repressive effect
on transcription, histone acetylation seems to have activating potential.
Physical activity is mentioned to be able to influence epigenetic modifications
in various tissues including NK-cells. In the present study we try to
find out if physical activity induced epigenetic modifications also affect
NK-cells of cancer patients.
To investigate the influence of physical activity on epigenetic modifications
in NK-cells, 8 female breast cancer patients in follow-up care perform
endurance training for 5 months. Before and after the intervention,
blood is taken and NK-cells are isolated using magnetic beads. Changes
in global DNA metyhylation and histone acetylation (H4K5) are detected
by immunocytochemistry, followed by optic density analysis (ImmageJ).
Data collection will be finished in October. We expect a lower DNA methylation
and a higher histone acetylation. Their impact on transcription is
oppositional and this result would promote a higher transcription. A higher
level of transcription could enhance the NK-cell activity by increasing
the receptor density.
If we can show a change in epigenetic modifications by enhanced physical
activity, a next step would be to investigate more precisely where
these changes are and how they influence the NK-cell activity in detail
Titel31. Deutscher Krebskongress : iKON - intelligente Konzepte in der Onkologie
ISBN (Print)ISSN 2296-5270
ISBN (elektronisch)e-ISSN 2296-5262
PublikationsstatusVeröffentlicht - 01.02.2014
VeranstaltungDeutscher Krebskongress - Berlin, Deutschland
Dauer: 19.08.201422.08.2014
Konferenznummer: 31