Detection method for recombinant human growth hormone based on 2D-PAGE

K. Püschel, Wilhelm Schänzer, Mario Thevis, M. Kohler, D. Sakharov, A. Tonevitskiy

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitrag - Aufsatz in KonferenzbandForschung


Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat
TitelRecent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008
Redakteure/-innenWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Herausgeber (Verlag)Sportverlag Strauß
PublikationsstatusVeröffentlicht - 2008
VeranstaltungCologne Workshop on Dope Analysis - Köln, Deutschland
Dauer: 24.02.200829.02.2008
Konferenznummer: 26

Fachgebiete und Schlagwörter

  • Biochemie
  • Blutplasma
  • Doping
  • Dopingnachweis
  • Sportmedizin
  • Untersuchungsmethode
  • Wachstumshormon