Detection method for recombinant human growth hormone based on 2D-PAGE

K. Püschel; Wilhelm Schänzer; Mario Thevis; M. Kohler; D. Sakharov; A. Tonevitskiy

Detection method for recombinant human growth hormone based on 2D-PAGE

K. Püschel, Wilhelm Schänzer, Mario Thevis, M. Kohler, D. Sakharov, A. Tonevitskiy

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

Abstract

Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat

Originalsprache	Deutsch
Titel	Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008
Herausgeber*innen	Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang	11
Herausgeber (Verlag)	Sportverlag Strauß
Erscheinungsdatum	2008
Seiten	233-243
Publikationsstatus	Veröffentlicht - 2008
Veranstaltung	Cologne Workshop on Dope Analysis - Köln, Deutschland Dauer: 24.02.2008 → 29.02.2008 Konferenznummer: 26

Fachgebiete und Schlagwörter

Biochemie
Blutplasma
Doping
Dopingnachweis
Sportmedizin
Untersuchungsmethode
Wachstumshormon

Zugriff auf Dokument

http://www.sport-if.de/dokumente/Doping_Analysis/2008/Articles/pp233-234.pdf

Zitation

Püschel, K., Schänzer, W., Thevis, M., Kohler, M., Sakharov, D., & Tonevitskiy, A. (2008). Detection method for recombinant human growth hormone based on 2D-PAGE. in W. Schänzer, H. Geyer, A. Gotzmann, & U. Mareck (Hrsg.), Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008 (S. 233-243). (Recent advances in doping analysis; Band 16). Sportverlag Strauß. http://www.sport-if.de/dokumente/Doping_Analysis/2008/Articles/pp233-234.pdf

Püschel, K. ; Schänzer, Wilhelm ; Thevis, Mario et al. / Detection method for recombinant human growth hormone based on 2D-PAGE. Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008. Hrsg. / Wilhelm Schänzer ; Hans Geyer ; A. Gotzmann ; Ute Mareck. Sportverlag Strauß, 2008. S. 233-243 (Recent advances in doping analysis).

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title = "Detection method for recombinant human growth hormone based on 2D-PAGE",

abstract = "Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat",

keywords = "Biochemie, Blutplasma, Doping, Dopingnachweis, Sportmedizin, Untersuchungsmethode, Wachstumshormon",

author = "K. P{\"u}schel and Wilhelm Sch{\"a}nzer and Mario Thevis and M. Kohler and D. Sakharov and A. Tonevitskiy",

year = "2008",

language = "Deutsch",

series = "Recent advances in doping analysis",

publisher = "Sportverlag Strau{\ss}",

pages = "233--243",

editor = "Wilhelm Sch{\"a}nzer and Hans Geyer and A. Gotzmann and Ute Mareck",

booktitle = "Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008",

note = "Cologne Workshop on Dope Analysis ; Conference date: 24-02-2008 Through 29-02-2008",

}

Püschel, K, Schänzer, W , Thevis, M, Kohler, M, Sakharov, D & Tonevitskiy, A 2008, Detection method for recombinant human growth hormone based on 2D-PAGE. in W Schänzer, H Geyer, A Gotzmann & U Mareck (Hrsg.), Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008. Recent advances in doping analysis, Bd. 16, Sportverlag Strauß, S. 233-243, Cologne Workshop on Dope Analysis, Köln, Deutschland, 24.02.08. <http://www.sport-if.de/dokumente/Doping_Analysis/2008/Articles/pp233-234.pdf>

Detection method for recombinant human growth hormone based on 2D-PAGE. / Püschel, K.; Schänzer, Wilhelm ; Thevis, Mario et al.
Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008. Hrsg. / Wilhelm Schänzer; Hans Geyer; A. Gotzmann; Ute Mareck. Sportverlag Strauß, 2008. S. 233-243 (Recent advances in doping analysis; Band 16).

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

TY - CHAP

T1 - Detection method for recombinant human growth hormone based on 2D-PAGE

AU - Püschel, K.

AU - Schänzer, Wilhelm

AU - Thevis, Mario

AU - Kohler, M.

AU - Sakharov, D.

AU - Tonevitskiy, A.

N1 - Conference code: 26

PY - 2008

Y1 - 2008

N2 - Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat

AB - Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat

KW - Biochemie

KW - Blutplasma

KW - Doping

KW - Dopingnachweis

KW - Sportmedizin

KW - Untersuchungsmethode

KW - Wachstumshormon

M3 - Konferenzbeitrag - Aufsatz in Konferenzband

T3 - Recent advances in doping analysis

SP - 233

EP - 243

BT - Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008

A2 - Schänzer, Wilhelm

A2 - Geyer, Hans

A2 - Gotzmann, A.

A2 - Mareck, Ute

PB - Sportverlag Strauß

T2 - Cologne Workshop on Dope Analysis

Y2 - 24 February 2008 through 29 February 2008

ER -

Püschel K, Schänzer W , Thevis M, Kohler M, Sakharov D, Tonevitskiy A. Detection method for recombinant human growth hormone based on 2D-PAGE. in Schänzer W, Geyer H, Gotzmann A, Mareck U, Hrsg., Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008. Sportverlag Strauß. 2008. S. 233-243. (Recent advances in doping analysis).