Doping control screening procedure for trenbolone analogues in human urine by LC-(APCI)-MS/MS

In the recent years the misuse of synthetically modified steroids (designer steroids) has been reported in professional sport repeatedly. The virtually unlimited variety of these compounds places a challenge for target analysis in doping control. One class of substances based on a 4,9,11-triene steroidal structure with a methyl residue at C-13 and different modifications at C-17 are trenbolone analogues, providing anabolic properties, and, thus are prohibited in sport according to regulations of the World-Anti-Doping-Agency (WADA). To improve screening for new target substances in doping control a qualitative assay based on LC-(APCI)-MS/MS was developed enabling the sensitive and selective detection of trenbolone (17ß-hydroxy-estra-4,9,11-trien-3-one), epitrenbolone (17α-hydroxy-estra-4,9,11-trien-3-one) and the chemically modified analogues methyltrenbolone (17ß-hydroxy-17-methylestra-4,9,11-trien-3-one), ethyltrenbolone (17ß-hydroxy-17-ethylestra-4,9,11-trien-3-one), propyltrenbolone(17ß-hydroxy-l7-propylestra-4,9,11-trien-3-one), "17-ketotrenbolone" (17-oxo-estra-4,9,11-trien-3-one) and altrenogest (17ß-hydroxy-17-(2-propen-1-yl)estra-4,9,11-trien-3-one) in human urine. Aus dem Text (geändert)