Establishment and characterization of a mouse embryonic heart slice preparation

Frank Pillekamp; Michael Reppel; Vera Dinkelacker; Yaqi Duan; Nathalie Jazmati; Wilhelm Bloch; Konrad Brockmeier; Juergen Hescheler; Bernd K Fleischmann; Ruediger Koehling

doi:10.1159/000087739

Establishment and characterization of a mouse embryonic heart slice preparation

Frank Pillekamp, Michael Reppel, Vera Dinkelacker, Yaqi Duan, Nathalie Jazmati, Wilhelm Bloch, Konrad Brockmeier, Juergen Hescheler, Bernd K Fleischmann, Ruediger Koehling

Abteilung Molekulare und zelluläre Sportmedizin

Universitätsklinikum Köln

Publikation: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung

Abstract

BACKGROUND: In contrast to isolated cells, the anatomic and functional integrity of tissue slices remains preserved. Aim of the study was to establish the slice technique in embryonic mouse hearts in order to perform physiological and pharmacological investigations of wild-type mice and genetically engineered mouse models of heart disease.

METHODS: Ventricular slices (thickness: 300 mum) were cut from agar-embedded embryonic mouse hearts (ED 16.5-18.5) with a vibratome. Histology, immunostaining with markers for apoptosis induction, intracellular recordings with sharp electrodes and field potential recordings using microelectrode arrays were performed to assess viability.

RESULTS: Slices exhibited normal histology without prominent signs of apoptosis for at least 24 hours. Intracellular recordings revealed the typical electrophysiological fingerprint of ventricular cardiomyocytes. Field potential recordings proved that adrenergic and muscarinic signaling was preserved.

CONCLUSION: Functionally intact heart slices can be generated from murine embryos.

Originalsprache	Englisch
Zeitschrift	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Jahrgang	16
Ausgabenummer	1-3
Seiten (von - bis)	127-132
Seitenumfang	6
ISSN	1015-8987
DOIs	https://doi.org/10.1159/000087739
Publikationsstatus	Veröffentlicht - 01.01.2005

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10.1159/000087739

Zitation

Pillekamp, F., Reppel, M., Dinkelacker, V., Duan, Y., Jazmati, N., Bloch, W., Brockmeier, K., Hescheler, J., Fleischmann, B. K., & Koehling, R. (2005). Establishment and characterization of a mouse embryonic heart slice preparation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 16(1-3), 127-132. https://doi.org/10.1159/000087739

@article{eb90a8b28a34455194e59ad2f18234ea,

title = "Establishment and characterization of a mouse embryonic heart slice preparation",

abstract = "BACKGROUND: In contrast to isolated cells, the anatomic and functional integrity of tissue slices remains preserved. Aim of the study was to establish the slice technique in embryonic mouse hearts in order to perform physiological and pharmacological investigations of wild-type mice and genetically engineered mouse models of heart disease.METHODS: Ventricular slices (thickness: 300 mum) were cut from agar-embedded embryonic mouse hearts (ED 16.5-18.5) with a vibratome. Histology, immunostaining with markers for apoptosis induction, intracellular recordings with sharp electrodes and field potential recordings using microelectrode arrays were performed to assess viability.RESULTS: Slices exhibited normal histology without prominent signs of apoptosis for at least 24 hours. Intracellular recordings revealed the typical electrophysiological fingerprint of ventricular cardiomyocytes. Field potential recordings proved that adrenergic and muscarinic signaling was preserved.CONCLUSION: Functionally intact heart slices can be generated from murine embryos.",

keywords = "Action Potentials, Animals, Apoptosis, Carbachol, Electrophysiology, Fetal Heart, Heart Rate, Isoproterenol, Mice, Staining and Labeling, Tissue Culture Techniques",

author = "Frank Pillekamp and Michael Reppel and Vera Dinkelacker and Yaqi Duan and Nathalie Jazmati and Wilhelm Bloch and Konrad Brockmeier and Juergen Hescheler and Fleischmann, {Bernd K} and Ruediger Koehling",

year = "2005",

month = jan,

day = "1",

doi = "10.1159/000087739",

language = "English",

volume = "16",

pages = "127--132",

journal = "Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology",

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Pillekamp, F, Reppel, M, Dinkelacker, V, Duan, Y, Jazmati, N, Bloch, W, Brockmeier, K, Hescheler, J, Fleischmann, BK & Koehling, R 2005, 'Establishment and characterization of a mouse embryonic heart slice preparation', Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Jg. 16, Nr. 1-3, S. 127-132. https://doi.org/10.1159/000087739

Establishment and characterization of a mouse embryonic heart slice preparation. / Pillekamp, Frank; Reppel, Michael; Dinkelacker, Vera et al.
in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Jahrgang 16, Nr. 1-3, 01.01.2005, S. 127-132.

Publikation: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung

TY - JOUR

T1 - Establishment and characterization of a mouse embryonic heart slice preparation

AU - Pillekamp, Frank

AU - Reppel, Michael

AU - Dinkelacker, Vera

AU - Duan, Yaqi

AU - Jazmati, Nathalie

AU - Bloch, Wilhelm

AU - Brockmeier, Konrad

AU - Hescheler, Juergen

AU - Fleischmann, Bernd K

AU - Koehling, Ruediger

PY - 2005/1/1

Y1 - 2005/1/1

N2 - BACKGROUND: In contrast to isolated cells, the anatomic and functional integrity of tissue slices remains preserved. Aim of the study was to establish the slice technique in embryonic mouse hearts in order to perform physiological and pharmacological investigations of wild-type mice and genetically engineered mouse models of heart disease.METHODS: Ventricular slices (thickness: 300 mum) were cut from agar-embedded embryonic mouse hearts (ED 16.5-18.5) with a vibratome. Histology, immunostaining with markers for apoptosis induction, intracellular recordings with sharp electrodes and field potential recordings using microelectrode arrays were performed to assess viability.RESULTS: Slices exhibited normal histology without prominent signs of apoptosis for at least 24 hours. Intracellular recordings revealed the typical electrophysiological fingerprint of ventricular cardiomyocytes. Field potential recordings proved that adrenergic and muscarinic signaling was preserved.CONCLUSION: Functionally intact heart slices can be generated from murine embryos.

AB - BACKGROUND: In contrast to isolated cells, the anatomic and functional integrity of tissue slices remains preserved. Aim of the study was to establish the slice technique in embryonic mouse hearts in order to perform physiological and pharmacological investigations of wild-type mice and genetically engineered mouse models of heart disease.METHODS: Ventricular slices (thickness: 300 mum) were cut from agar-embedded embryonic mouse hearts (ED 16.5-18.5) with a vibratome. Histology, immunostaining with markers for apoptosis induction, intracellular recordings with sharp electrodes and field potential recordings using microelectrode arrays were performed to assess viability.RESULTS: Slices exhibited normal histology without prominent signs of apoptosis for at least 24 hours. Intracellular recordings revealed the typical electrophysiological fingerprint of ventricular cardiomyocytes. Field potential recordings proved that adrenergic and muscarinic signaling was preserved.CONCLUSION: Functionally intact heart slices can be generated from murine embryos.

KW - Action Potentials

KW - Animals

KW - Apoptosis

KW - Carbachol

KW - Electrophysiology

KW - Fetal Heart

KW - Heart Rate

KW - Isoproterenol

KW - Mice

KW - Staining and Labeling

KW - Tissue Culture Techniques

U2 - 10.1159/000087739

DO - 10.1159/000087739

M3 - Journal articles

C2 - 16121041

SN - 1015-8987

VL - 16

SP - 127

EP - 132

JO - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

IS - 1-3

ER -

Establishment and characterization of a mouse embryonic heart slice preparation

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