Fibroblasts support functional integration of purified embryonic stem cell-derived cardiomyocytes into avital myocardial tissue

Jiaoya Xi, Markus Khalil, Dimitry Spitkovsky, Tobias Hannes, Kurt Pfannkuche, Wilhelm Bloch, Tomo Sarić, Konrad Brockmeier, Juergen Hescheler, Frank Pillekamp

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Abstract

Transplantation of purified pluripotent stem cell-derived cardiomyocytes into damaged myocardium might become a therapy to improve contractile function after myocardial infarction. However, engraftment remains problematic. Aim of this study was to investigate whether murine embryonic fibroblasts (MEFs) support the functional integration of purified embryonic stem cell-derived cardiomyocytes (ES-CMs). Neonatal murine ventricular tissue slices were subjected to oxygen and glucose deprivation to simulate irreversible ischemia. Vital tissue slices served as control. Vital and avital tissue slices were cultured with or without MEFs before coculturing with clusters of puromycin-selected ES-CMs. Integration of ES-CM clusters was assessed morphologically, motility by long-term microscopy, and functional integration by isometric force measurements. We observed a good morphological integration into vital but a poor integration into avital slices. Adding MEFs improved morphological integration into irreversibly damaged slices and enabled purified ES-CMs to migrate and to confer force. We conclude that noncardiomyocytes like MEFs support morphological integration and force transmission of purified ES-CMs by enabling adhesion and migration.

OriginalspracheEnglisch
ZeitschriftStem cells and development
Jahrgang20
Ausgabenummer5
Seiten (von - bis)821-830
Seitenumfang10
ISSN1547-3287
DOIs
PublikationsstatusVeröffentlicht - 01.05.2011

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