Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS

Anne-Katrin Orlovius; Sven Guddat; Maria Kristina Parr; A. Koch; M. Gütschow; Mario Thevis; Wilhelm Schänzer

Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS

Anne-Katrin Orlovius, Sven Guddat, Maria Kristina Parr, A. Koch, M. Gütschow, Mario Thevis, Wilhelm Schänzer

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

Abstract

Herein we report the synthesis and characterization of the sulfoconjugate of octopamine and the excretion profile of the sulfoconjugated compound compared to the unchanged drug. The evaded hydrolysis of the compounds enables combination with other substances in screening procedures: for example direct determination of intact sulfoconjugates in combination with diuretics, stimulants, β2-agonists, plasma volume expanders and narcotics without any sample preparation except addition of internal standard. In order to investigate the intact conjugate of octopamine as well as the unchanged therapeutic agent and phase-I metabolites, urine samples were extracted by means of solid phase extraction (SPE) and subsequently analyzed by liquid chromatography tandem mass spectrometry. The employed assay was validated including intra-day and inter-day precision, limit of detection, specificity and recovery. Further, post-administration urine samples were analyzed according to described sample preparation and evaluated concerning the quantity of octopamine and its sulfoconjugate. Einleitung gekürzt

Originalsprache	Englisch
Titel	Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011
Herausgeber*innen	Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang	9
Herausgeber (Verlag)	Sportverlag Strauß
Erscheinungsdatum	2011
Seiten	34-42
Publikationsstatus	Veröffentlicht - 2011
Veranstaltung	Cologne Workshop on Dope Analysis - Köln, Deutschland Dauer: 13.02.2011 → 18.02.2011 Konferenznummer: 29

Fachgebiete und Schlagwörter

Biochemie
Doping
Dopinganalyse
Dopingbekämpfung
Dopingnachweis
Massenspektrometrie
Sportmedizin
Stimulans
Synthese
Urinuntersuchung

Zitation

Orlovius, A-K., Guddat, S., Parr, M. K., Koch, A., Gütschow, M., Thevis, M., & Schänzer, W. (2011). Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS. in W. Schänzer, H. Geyer, A. Gotzmann, & U. Mareck (Hrsg.), Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011 (S. 34-42). (Recent advances in doping analysis; Band 19). Sportverlag Strauß.

Orlovius, Anne-Katrin ; Guddat, Sven ; Parr, Maria Kristina et al. / Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS. Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011. Hrsg. / Wilhelm Schänzer ; Hans Geyer ; A. Gotzmann ; Ute Mareck. Sportverlag Strauß, 2011. S. 34-42 (Recent advances in doping analysis).

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title = "Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS",

abstract = "Herein we report the synthesis and characterization of the sulfoconjugate of octopamine and the excretion profile of the sulfoconjugated compound compared to the unchanged drug. The evaded hydrolysis of the compounds enables combination with other substances in screening procedures: for example direct determination of intact sulfoconjugates in combination with diuretics, stimulants, β2-agonists, plasma volume expanders and narcotics without any sample preparation except addition of internal standard. In order to investigate the intact conjugate of octopamine as well as the unchanged therapeutic agent and phase-I metabolites, urine samples were extracted by means of solid phase extraction (SPE) and subsequently analyzed by liquid chromatography tandem mass spectrometry. The employed assay was validated including intra-day and inter-day precision, limit of detection, specificity and recovery. Further, post-administration urine samples were analyzed according to described sample preparation and evaluated concerning the quantity of octopamine and its sulfoconjugate. Einleitung gek{\"u}rzt",

keywords = "Biochemie, Doping, Dopinganalyse, Dopingbek{\"a}mpfung, Dopingnachweis, Massenspektrometrie, Sportmedizin, Stimulans, Synthese, Urinuntersuchung",

author = "Anne-Katrin Orlovius and Sven Guddat and Parr, {Maria Kristina} and A. Koch and M. G{\"u}tschow and Mario Thevis and Wilhelm Sch{\"a}nzer",

year = "2011",

language = "English",

series = "Recent advances in doping analysis",

publisher = "Sportverlag Strau{\ss}",

pages = "34--42",

editor = "Wilhelm Sch{\"a}nzer and Hans Geyer and A. Gotzmann and Ute Mareck",

booktitle = "Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011",

note = "Cologne Workshop on Dope Analysis ; Conference date: 13-02-2011 Through 18-02-2011",

}

Orlovius, A-K , Guddat, S, Parr, MK, Koch, A, Gütschow, M, Thevis, M & Schänzer, W 2011, Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS. in W Schänzer, H Geyer, A Gotzmann & U Mareck (Hrsg.), Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011. Recent advances in doping analysis, Bd. 19, Sportverlag Strauß, S. 34-42, Cologne Workshop on Dope Analysis , Köln, Deutschland, 13.02.11.

Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS. / Orlovius, Anne-Katrin ; Guddat, Sven; Parr, Maria Kristina et al.
Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011. Hrsg. / Wilhelm Schänzer; Hans Geyer; A. Gotzmann; Ute Mareck. Sportverlag Strauß, 2011. S. 34-42 (Recent advances in doping analysis; Band 19).

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

TY - CHAP

T1 - Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS

AU - Orlovius, Anne-Katrin

AU - Guddat, Sven

AU - Parr, Maria Kristina

AU - Koch, A.

AU - Gütschow, M.

AU - Thevis, Mario

AU - Schänzer, Wilhelm

N1 - Conference code: 29

PY - 2011

Y1 - 2011

N2 - Herein we report the synthesis and characterization of the sulfoconjugate of octopamine and the excretion profile of the sulfoconjugated compound compared to the unchanged drug. The evaded hydrolysis of the compounds enables combination with other substances in screening procedures: for example direct determination of intact sulfoconjugates in combination with diuretics, stimulants, β2-agonists, plasma volume expanders and narcotics without any sample preparation except addition of internal standard. In order to investigate the intact conjugate of octopamine as well as the unchanged therapeutic agent and phase-I metabolites, urine samples were extracted by means of solid phase extraction (SPE) and subsequently analyzed by liquid chromatography tandem mass spectrometry. The employed assay was validated including intra-day and inter-day precision, limit of detection, specificity and recovery. Further, post-administration urine samples were analyzed according to described sample preparation and evaluated concerning the quantity of octopamine and its sulfoconjugate. Einleitung gekürzt

AB - Herein we report the synthesis and characterization of the sulfoconjugate of octopamine and the excretion profile of the sulfoconjugated compound compared to the unchanged drug. The evaded hydrolysis of the compounds enables combination with other substances in screening procedures: for example direct determination of intact sulfoconjugates in combination with diuretics, stimulants, β2-agonists, plasma volume expanders and narcotics without any sample preparation except addition of internal standard. In order to investigate the intact conjugate of octopamine as well as the unchanged therapeutic agent and phase-I metabolites, urine samples were extracted by means of solid phase extraction (SPE) and subsequently analyzed by liquid chromatography tandem mass spectrometry. The employed assay was validated including intra-day and inter-day precision, limit of detection, specificity and recovery. Further, post-administration urine samples were analyzed according to described sample preparation and evaluated concerning the quantity of octopamine and its sulfoconjugate. Einleitung gekürzt

KW - Biochemie

KW - Doping

KW - Dopinganalyse

KW - Dopingbekämpfung

KW - Dopingnachweis

KW - Massenspektrometrie

KW - Sportmedizin

KW - Stimulans

KW - Synthese

KW - Urinuntersuchung

M3 - Conference contribution - Article for conference

T3 - Recent advances in doping analysis

SP - 34

EP - 42

BT - Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011

A2 - Schänzer, Wilhelm

A2 - Geyer, Hans

A2 - Gotzmann, A.

A2 - Mareck, Ute

PB - Sportverlag Strauß

T2 - Cologne Workshop on Dope Analysis

Y2 - 13 February 2011 through 18 February 2011

ER -

Orlovius A-K , Guddat S, Parr MK, Koch A, Gütschow M, Thevis M et al. Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS. in Schänzer W, Geyer H, Gotzmann A, Mareck U, Hrsg., Recent advances in doping analysis (19) : Proceedings of the Manfred-Donike-Workshop, 29th Cologne Workshop on Dope Analysis : 13th to 18th February 2011. Sportverlag Strauß. 2011. S. 34-42. (Recent advances in doping analysis).

Identification and monitoring of octopamine sulfoconjugate in urine by LC/(ESI)-MS/MS

Abstract

Fachgebiete und Schlagwörter

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