Long-term effects of dietary isoflavones on uterine gene expression profiles

Frank Josef Möller, Oliver Zierau, Torsten Hertrampf, Anja Bliedtner, Patrick Rene Diel, Günter Vollmer

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Abstract

Isoflavones (ISOs) are bioactive food ingredients of the traditional East Asian diet and currently discussed as alternatives to classical hormone replacement therapies and for reducing the prevalence of hormone-dependent cancers. Although there are many studies on ISOs, not much is known about their long-term effects. Therefore, we performed an animal experiment analyzing the effects of three different diets: a phytoestrogen-free diet, a diet supplemented with genistein (700 microg/g diet) and an ISO-high diet (232 microg daidzein and 240 microg genistein/g) at two distinct time points, juvenile (21 days) and adult (97 days). Exposure started prior to mating of the parents and throughout the life of the offspring. We observed a stronger increase of uterine wet weights in juvenile offspring with genistein exposure (1018+/-350 mg/kg BW) than with ISO-high diet (497+/-133 mg/kg BW). Whereas the expression of proliferation related genes (PCNA; Ki67; IGF-1; IGF-1R), analyzed by real-time-qPCR and Western blot, were significantly down-regulated in juvenile animals exposed to genistein. Additionally, genistein exposure led to estrogenic responses, observed upon increase of complement C3 and decrease of estrogen receptors gene expressions, while the exposure to ISO-high diet did not show these effects. In conclusion, both the time point on which phytoestrogen exposure starts together with the composition of the ingested phytoestrogen containing diet are of great importance for the biological response of the offspring.

OriginalspracheEnglisch
ZeitschriftThe Journal of Steroid Biochemistry and Molecular Biology
Jahrgang113
Ausgabenummer3-5
Seiten (von - bis)296-303
Seitenumfang8
ISSN0960-0760
DOIs
PublikationsstatusVeröffentlicht - 01.02.2009

Fingerprint

Untersuchen Sie die Forschungsthemen von „Long-term effects of dietary isoflavones on uterine gene expression profiles“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitation