Mass spectrometric identification of human pituitary growth hormone variants

Maxie Kohler, Klaus Püschel, Wilhelm Schänzer, Mario Thevis

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitrag - Aufsatz in KonferenzbandForschung

Abstract

Detailed analysis and characterization of growth hormone in blood and pituitary shall provide structural information on different variants and may demonstrate connections between the appearance of isoforms and special metabolic states or conditions. The identification of additional fragments may also complement the knowledge about differences between recombinant and endogenous human growth hormone and provide targets for future drug testing assays. Hence, in the present work human pituitary growth hormone was analyzed by two dimensional gel electrophoresis and orbitrap mass spectrometry aflter trypsin digestion of Coomassie blue stained spots. The variants of human pituitary growth hormone detected here show only a small part of the heterogeneity, and single spots may contain a far more complex set of modifications. In this work phophorylations were found to be responsible for pI shift to more acidic pH. Tryptophan residues were found to be oxidized in several growth hormone variants and oxidation states were interindividually different. Furthermore, dimeric forms of human growth hormone were only found in some of the analyzed pituitaries. Isoforms that appear interindividually different should be studied in detail because changes in the three dimensional structure may influence the binding properties in antibody based assays. For anti-doping purposes, it will be essential to compare the variants found in the pituitary extracts and plasma samples. Aus dem Text
OriginalspracheEnglisch
TitelRecent advances in doping analysis (15) : Proceedings of the Manfred Donike Workshop ; 25th Cologne Workshop on Dope Analysis 25th February to 2nd March 2007
Herausgeber*innenWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang10
Herausgeber (Verlag)Sportverlag Strauß
Erscheinungsdatum2007
Seiten21-30
PublikationsstatusVeröffentlicht - 2007
VeranstaltungCologne Workshop on Dope Analysis - Köln, Deutschland
Dauer: 25.02.200702.03.2007
Konferenznummer: 25

Fachgebiete und Schlagwörter

  • Biochemie
  • Doping
  • Dopingnachweis
  • Elektrophorese
  • Hypophysenhormon
  • Leistungssport
  • Leistungssteigerung
  • Massenspektrometrie
  • Messverfahren
  • Sportmedizin
  • Untersuchungsmethode
  • Wachstumshormon

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