New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS

Gregor Fußhöller; A. Koch; U. Mareck; Andreas Thomas; A. Schmechel; G. Sigmund; Mario Thevis; Wilhelm Schänzer

New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS

Gregor Fußhöller, A. Koch, U. Mareck, Andreas Thomas, A. Schmechel, G. Sigmund, Mario Thevis, Wilhelm Schänzer

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

Abstract

This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3’-OH-stanozolol, 4ß-OH-stanozolol and 16ß-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4ß-OH-stanozolol and 16ß-OH-stanozolol. Aus dem Text

Originalsprache	Englisch
Titel	Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006
Herausgeber*innen	Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang	10
Herausgeber (Verlag)	Sport & Buch Strauß
Erscheinungsdatum	2006
Seiten	229-238
Publikationsstatus	Veröffentlicht - 2006
Veranstaltung	Cologne Workshop on Dope Analysis - Köln, Deutschland Dauer: 04.06.2006 → 09.06.2006 Konferenznummer: 24

Fachgebiete und Schlagwörter

Biochemie
Doping
Dopingnachweis
Flüssigkeitschromatographie
Gaschromatographie
Leistungssport
Leistungssteigerung
Massenspektrometrie
Messverfahren
Metabolit
Sportmedizin
Steroid, anaboles
Untersuchungsmethode
Urin
Urinuntersuchung

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http://www.sport-if.de/dokumente/Doping_Analysis/2006/articles/pp229-238.pdf

Zitation

Fußhöller, G., Koch, A., Mareck, U., Thomas, A., Schmechel, A., Sigmund, G., Thevis, M., & Schänzer, W. (2006). New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS. in W. Schänzer, H. Geyer, A. Gotzmann, & U. Mareck (Hrsg.), Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006 (S. 229-238). (Recent advances in doping analysis; Band 14). Sport & Buch Strauß. http://www.sport-if.de/dokumente/Doping_Analysis/2006/articles/pp229-238.pdf

Fußhöller, Gregor ; Koch, A. ; Mareck, U. et al. / New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS. Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006. Hrsg. / Wilhelm Schänzer ; Hans Geyer ; A. Gotzmann ; Ute Mareck. Sport & Buch Strauß, 2006. S. 229-238 (Recent advances in doping analysis).

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title = "New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS",

abstract = "This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3{\textquoteright}-OH-stanozolol, 4{\ss}-OH-stanozolol and 16{\ss}-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4{\ss}-OH-stanozolol and 16{\ss}-OH-stanozolol. Aus dem Text",

keywords = "Biochemie, Doping, Dopingnachweis, Fl{\"u}ssigkeitschromatographie, Gaschromatographie, Leistungssport, Leistungssteigerung, Massenspektrometrie, Messverfahren, Metabolit, Sportmedizin, Steroid, anaboles, Untersuchungsmethode, Urin, Urinuntersuchung",

author = "Gregor Fu{\ss}h{\"o}ller and A. Koch and U. Mareck and Andreas Thomas and A. Schmechel and G. Sigmund and Mario Thevis and Wilhelm Sch{\"a}nzer",

year = "2006",

language = "English",

series = "Recent advances in doping analysis",

publisher = "Sport & Buch Strau{\ss}",

pages = "229--238",

editor = "Wilhelm Sch{\"a}nzer and Hans Geyer and A. Gotzmann and Ute Mareck",

booktitle = "Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006",

note = "Cologne Workshop on Dope Analysis ; Conference date: 04-06-2006 Through 09-06-2006",

}

Fußhöller, G, Koch, A, Mareck, U, Thomas, A, Schmechel, A, Sigmund, G, Thevis, M & Schänzer, W 2006, New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS. in W Schänzer, H Geyer, A Gotzmann & U Mareck (Hrsg.), Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006. Recent advances in doping analysis, Bd. 14, Sport & Buch Strauß, S. 229-238, Cologne Workshop on Dope Analysis, Köln, Deutschland, 04.06.06. <http://www.sport-if.de/dokumente/Doping_Analysis/2006/articles/pp229-238.pdf>

New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS. / Fußhöller, Gregor; Koch, A.; Mareck, U. et al.
Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006. Hrsg. / Wilhelm Schänzer; Hans Geyer; A. Gotzmann; Ute Mareck. Sport & Buch Strauß, 2006. S. 229-238 (Recent advances in doping analysis; Band 14).

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung

TY - CHAP

T1 - New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS

AU - Fußhöller, Gregor

AU - Koch, A.

AU - Mareck, U.

AU - Thomas, Andreas

AU - Schmechel, A.

AU - Sigmund, G.

AU - Thevis, Mario

AU - Schänzer, Wilhelm

N1 - Conference code: 24

PY - 2006

Y1 - 2006

N2 - This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3’-OH-stanozolol, 4ß-OH-stanozolol and 16ß-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4ß-OH-stanozolol and 16ß-OH-stanozolol. Aus dem Text

AB - This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3’-OH-stanozolol, 4ß-OH-stanozolol and 16ß-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4ß-OH-stanozolol and 16ß-OH-stanozolol. Aus dem Text

KW - Biochemie

KW - Doping

KW - Dopingnachweis

KW - Flüssigkeitschromatographie

KW - Gaschromatographie

KW - Leistungssport

KW - Leistungssteigerung

KW - Massenspektrometrie

KW - Messverfahren

KW - Metabolit

KW - Sportmedizin

KW - Steroid, anaboles

KW - Untersuchungsmethode

KW - Urin

KW - Urinuntersuchung

M3 - Conference contribution - Article for conference

T3 - Recent advances in doping analysis

SP - 229

EP - 238

BT - Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006

A2 - Schänzer, Wilhelm

A2 - Geyer, Hans

A2 - Gotzmann, A.

A2 - Mareck, Ute

PB - Sport & Buch Strauß

T2 - Cologne Workshop on Dope Analysis

Y2 - 4 June 2006 through 9 June 2006

ER -

Fußhöller G, Koch A, Mareck U, Thomas A, Schmechel A, Sigmund G et al. New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS. in Schänzer W, Geyer H, Gotzmann A, Mareck U, Hrsg., Recent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006. Sport & Buch Strauß. 2006. S. 229-238. (Recent advances in doping analysis).

New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS

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