Preliminary results for the development of a peptide screening method by means of LC-MS/MS

Andreas Thomas; Maxie Kohler; Philippe Delahaut; Wilhelm Schänzer; Mario Thevis

Preliminary results for the development of a peptide screening method by means of LC-MS/MS

Andreas Thomas, Maxie Kohler, Philippe Delahaut, Wilhelm Schänzer, Mario Thevis

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung › Begutachtung

Abstract

Bioactive peptides such as insulins, synthetic adrenocorticotrophic hormone (ACTH) analogue Synacthen, Gonadorelin (LHRH) and insulin-like growth factors (IGF) provide a reasonable potential for the misuse as performance enhancing agents and are prohibited in elite sports according to the list of banned substances established by the WADA. Currently, determination of these target analytes is possible by single assays only. The present method provides results for a preliminary approach to determine various prohibited peptides occurring in urine (e.g. Gonadorelin, Humalog (Insulin Lispro), Apidra (Insulin Glulisine), Novolog (Insulin Aspart), Lantus (Insulin Glargine), Porcine Insulin, Bovine Insulin, IGF-1 etc.) in one screening procedure. The method enables the effective, highly sensitive and specific screening for several different target analytes that are simultaneously purified and analysed by means of immunoaffinity purification, subsequent liquid-chromatographic separation and high resolution / high accuracy mass spectrometric determination. Principally, the approach is extendable to any banned peptide, if adequate antibodies are available. At the present status of the project only a limited number of analytes were implemented in the method. Verf.-Referat

Originalsprache	Deutsch
Titel	Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010
Herausgeber*innen	Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang	9
Herausgeber (Verlag)	Sportverlag Strauß
Erscheinungsdatum	2010
Seiten	109-117
Publikationsstatus	Veröffentlicht - 2010
Veranstaltung	Cologne Workshop on Dope Analysis - Köln, Deutschland Dauer: 07.03.2010 → 12.03.2010 Konferenznummer: 28

Fachgebiete und Schlagwörter

Biochemie
Doping
Dopinganalyse
Flüssigkeitschromatographie
Massenspektrometrie
Methodik
Peptid
Screening
Sportmedizin
Testverfahren

Zitation

Thomas, A., Kohler, M., Delahaut, P., Schänzer, W., & Thevis, M. (2010). Preliminary results for the development of a peptide screening method by means of LC-MS/MS. in W. Schänzer, H. Geyer, A. Gotzmann, & U. Mareck (Hrsg.), Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010 (S. 109-117). (Recent advances in doping analysis; Band 18). Sportverlag Strauß.

Thomas, Andreas ; Kohler, Maxie ; Delahaut, Philippe et al. / Preliminary results for the development of a peptide screening method by means of LC-MS/MS. Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010. Hrsg. / Wilhelm Schänzer ; Hans Geyer ; A. Gotzmann ; Ute Mareck. Sportverlag Strauß, 2010. S. 109-117 (Recent advances in doping analysis).

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title = "Preliminary results for the development of a peptide screening method by means of LC-MS/MS",

abstract = "Bioactive peptides such as insulins, synthetic adrenocorticotrophic hormone (ACTH) analogue Synacthen, Gonadorelin (LHRH) and insulin-like growth factors (IGF) provide a reasonable potential for the misuse as performance enhancing agents and are prohibited in elite sports according to the list of banned substances established by the WADA. Currently, determination of these target analytes is possible by single assays only. The present method provides results for a preliminary approach to determine various prohibited peptides occurring in urine (e.g. Gonadorelin, Humalog (Insulin Lispro), Apidra (Insulin Glulisine), Novolog (Insulin Aspart), Lantus (Insulin Glargine), Porcine Insulin, Bovine Insulin, IGF-1 etc.) in one screening procedure. The method enables the effective, highly sensitive and specific screening for several different target analytes that are simultaneously purified and analysed by means of immunoaffinity purification, subsequent liquid-chromatographic separation and high resolution / high accuracy mass spectrometric determination. Principally, the approach is extendable to any banned peptide, if adequate antibodies are available. At the present status of the project only a limited number of analytes were implemented in the method. Verf.-Referat",

keywords = "Biochemie, Doping, Dopinganalyse, Fl{\"u}ssigkeitschromatographie, Massenspektrometrie, Methodik, Peptid, Screening, Sportmedizin, Testverfahren",

author = "Andreas Thomas and Maxie Kohler and Philippe Delahaut and Wilhelm Sch{\"a}nzer and Mario Thevis",

year = "2010",

language = "Deutsch",

series = "Recent advances in doping analysis",

publisher = "Sportverlag Strau{\ss}",

pages = "109--117",

editor = "Wilhelm Sch{\"a}nzer and Hans Geyer and A. Gotzmann and Ute Mareck",

booktitle = "Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010",

note = "Cologne Workshop on Dope Analysis ; Conference date: 07-03-2010 Through 12-03-2010",

}

Thomas, A, Kohler, M, Delahaut, P, Schänzer, W & Thevis, M 2010, Preliminary results for the development of a peptide screening method by means of LC-MS/MS. in W Schänzer, H Geyer, A Gotzmann & U Mareck (Hrsg.), Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010. Recent advances in doping analysis, Bd. 18, Sportverlag Strauß, S. 109-117, Cologne Workshop on Dope Analysis, Köln, Deutschland, 07.03.10.

Preliminary results for the development of a peptide screening method by means of LC-MS/MS. / Thomas, Andreas; Kohler, Maxie; Delahaut, Philippe et al.
Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010. Hrsg. / Wilhelm Schänzer; Hans Geyer; A. Gotzmann; Ute Mareck. Sportverlag Strauß, 2010. S. 109-117 (Recent advances in doping analysis; Band 18).

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag - Aufsatz in Konferenzband › Forschung › Begutachtung

TY - CHAP

T1 - Preliminary results for the development of a peptide screening method by means of LC-MS/MS

AU - Thomas, Andreas

AU - Kohler, Maxie

AU - Delahaut, Philippe

AU - Schänzer, Wilhelm

AU - Thevis, Mario

N1 - Conference code: 28

PY - 2010

Y1 - 2010

N2 - Bioactive peptides such as insulins, synthetic adrenocorticotrophic hormone (ACTH) analogue Synacthen, Gonadorelin (LHRH) and insulin-like growth factors (IGF) provide a reasonable potential for the misuse as performance enhancing agents and are prohibited in elite sports according to the list of banned substances established by the WADA. Currently, determination of these target analytes is possible by single assays only. The present method provides results for a preliminary approach to determine various prohibited peptides occurring in urine (e.g. Gonadorelin, Humalog (Insulin Lispro), Apidra (Insulin Glulisine), Novolog (Insulin Aspart), Lantus (Insulin Glargine), Porcine Insulin, Bovine Insulin, IGF-1 etc.) in one screening procedure. The method enables the effective, highly sensitive and specific screening for several different target analytes that are simultaneously purified and analysed by means of immunoaffinity purification, subsequent liquid-chromatographic separation and high resolution / high accuracy mass spectrometric determination. Principally, the approach is extendable to any banned peptide, if adequate antibodies are available. At the present status of the project only a limited number of analytes were implemented in the method. Verf.-Referat

AB - Bioactive peptides such as insulins, synthetic adrenocorticotrophic hormone (ACTH) analogue Synacthen, Gonadorelin (LHRH) and insulin-like growth factors (IGF) provide a reasonable potential for the misuse as performance enhancing agents and are prohibited in elite sports according to the list of banned substances established by the WADA. Currently, determination of these target analytes is possible by single assays only. The present method provides results for a preliminary approach to determine various prohibited peptides occurring in urine (e.g. Gonadorelin, Humalog (Insulin Lispro), Apidra (Insulin Glulisine), Novolog (Insulin Aspart), Lantus (Insulin Glargine), Porcine Insulin, Bovine Insulin, IGF-1 etc.) in one screening procedure. The method enables the effective, highly sensitive and specific screening for several different target analytes that are simultaneously purified and analysed by means of immunoaffinity purification, subsequent liquid-chromatographic separation and high resolution / high accuracy mass spectrometric determination. Principally, the approach is extendable to any banned peptide, if adequate antibodies are available. At the present status of the project only a limited number of analytes were implemented in the method. Verf.-Referat

KW - Biochemie

KW - Doping

KW - Dopinganalyse

KW - Flüssigkeitschromatographie

KW - Massenspektrometrie

KW - Methodik

KW - Peptid

KW - Screening

KW - Sportmedizin

KW - Testverfahren

M3 - Konferenzbeitrag - Aufsatz in Konferenzband

T3 - Recent advances in doping analysis

SP - 109

EP - 117

BT - Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010

A2 - Schänzer, Wilhelm

A2 - Geyer, Hans

A2 - Gotzmann, A.

A2 - Mareck, Ute

PB - Sportverlag Strauß

T2 - Cologne Workshop on Dope Analysis

Y2 - 7 March 2010 through 12 March 2010

ER -

Thomas A, Kohler M, Delahaut P, Schänzer W , Thevis M. Preliminary results for the development of a peptide screening method by means of LC-MS/MS. in Schänzer W, Geyer H, Gotzmann A, Mareck U, Hrsg., Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010. Sportverlag Strauß. 2010. S. 109-117. (Recent advances in doping analysis).