Abstract
In this work, a novel initial testing assay based on liquid chromatography-mass spectrometry is presented, enabling the detection of peptidic drugs and drug candidates (< 2 kDa) prohibited in sports. The assay covers representatives and metabolites of gonadotropin releasing hormone and its analogs (GnRHs), growth hormone secretagogues (GHS), growth hormone releasing peptides (GHRPs), and the Vasopressin-analog Desmopressin. The general objective of this work was to reduce sample preparation efforts to a minimum while preserving highest possible sensitivity and specificity of the assay, demonstrating limits of detection between 50 and 200 pg/mL. Here, a "dilute-and-inject" strategy provides the simplest conceivable sample preparation procedure. Furthermore, the combination of well-established strategies for the determination of peptides, such as two-dimensional liquid chromatography, dimethyl sulfoxide (DMSO)-assisted electrospray ionization, high resolution mass spectrometric detection and a tailored reporter template, which facilitates data review enormously, provides a high-throughput initial testing assay for lower molecular mass peptidic and peptide-related analytes.
Originalsprache | Englisch |
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Zeitschrift | Drug testing and analysis |
Jahrgang | 10 |
Ausgabenummer | 11-12 |
Seiten (von - bis) | 1755-1760 |
Seitenumfang | 6 |
ISSN | 1942-7603 |
DOIs | |
Publikationsstatus | Veröffentlicht - 21.09.2018 |
Veranstaltung | Manfred Donike Workshop on Doping Analysis - Köln, Deutschland Dauer: 23.04.2018 → 26.04.2018 Konferenznummer: 36 |