Terbutaline sulfoconjugate : characterization and urinary excretion monitored by LC/ESI-MS/MS

Titel in Übersetzung: Terbutalin Sulfatverbindungen : Beschreibung und Urinausscheidung überwacht durch LC/ESI-MS/MS

Anne-Katrin Orlovius, Sven Guddat, Maria Kristina Parr, Maxie Kohler, M. Gütschow, Mario Thevis, Wilhelm Schänzer

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung


Terbutaline is a fast-acting beta(2)-adrenergic agonist used in the treatment of obstructive pulmonary diseases. Doping control for beta(2)-agonists, which are forbidden in sports by the World Anti-doping Agency (WADA), is performed in screening by liquid chromatography/mass spectrometry after hydrolysis of phase-II metabolites. In this study, the mono-sulfoconjugated phase-II metabolite of terbutaline was synthesized and the chemical structure was characterized by (1)H-nuclear magnetic resonance spectrometry and high resolution/high accuracy Orbitrap mass spectrometry. The metabolite was designated as the phenolic esterified compound, which has been mentioned in most literature reports but has not been verified so far. The benzylic esterified compound was also synthesized and characterized by high-resolution/high accuracy Orbitrap mass spectrometry but was not detectable in urine samples from an excretion study performed after a single application of one terbutaline capsule (7.5 mg terbutaline sulfate salt). The phenolic sulfate of terbutaline was detected for two to four days after administration, whereas the unchanged terbutaline was detected for four to five days. A glucuronidated, disulfated or trisulfated phase-II metabolite of terbutaline was not found. The measurement of phase-II metabolites is planned to be incorporated into existing screening procedures to allow a faster sample preparation. Verf.-Referat
Titel in ÜbersetzungTerbutalin Sulfatverbindungen : Beschreibung und Urinausscheidung überwacht durch LC/ESI-MS/MS
ZeitschriftDrug testing and analysis
Seiten (von - bis)568-575
PublikationsstatusVeröffentlicht - 2009

Fachgebiete und Schlagwörter

  • Doping
  • Dopinganalyse
  • Dopingnachweis
  • Gaschromatographie
  • Massenspektrometrie
  • Methodik
  • Sulfat
  • Urin
  • Urinausscheidung
  • Urinuntersuchung