Chromatographic-mass spectrometric analysis of peptidic analytes (2-10 kDa) in doping control urine samples

Publication: Contribution to journalJournal articlesResearchpeer-review


Peptides with a molecular mass between 2 and 10 kDa that are prohibited in elite sports usually require dedicated sample preparation and mass spectrometric detection that commonly cannot be combined with other (lower molecular mass) substances. In most instances, the physicochemical differences are too significant to allow for a generic analytical procedure. A simplification of established and comparably complex analytical approaches is therefore desirable and has been accomplished in the context of this study. With urine samples representing still the most frequently collected doping control specimens, efficient extraction of peptidic analytes from this matrix was a major goal of this method, as demonstrated for the included compounds such as insulins (human, lispro, aspart, glulisine, tresiba, glargine metabolite, bovine insulin, porcine insulin), growth hormone-releasing hormones (sermorelin, CJC-1295, tesamorelin) incl. their respective metabolites, insulin-like-growth factors (long-R3 -IGF-I, R3 -IGF-I, des1-3 -IGF-I), synacthen, gonadorelin and mechano growth factors (human MGF, MGF-Goldspink). Sample preparation and detection are controlled by five internal standards, covering all five included peptide drug categories. Nearly all requirements of the recent technical documents from the World Anti-Doping Agency (WADA) considering their minimum required performance levels (MRPL) are fulfilled, and the method was validated for its utilisation as initial testing procedure in doping controls. Finally, the approach was applied to authentic post-administration study urine samples (for insulins and gonadorelin) in order to provide proof of principle.

Original languageEnglish
Article numbere4996
JournalJournal of mass spectrometry : JMS
Issue number1
Publication statusPublished - 01.01.2024

Research areas and keywords

  • Humans
  • Swine
  • Animals
  • Cattle
  • Insulin-Like Growth Factor I
  • Doping in Sports
  • Body Fluids
  • Gonadotropin-Releasing Hormone
  • Insulin


Dive into the research topics of 'Chromatographic-mass spectrometric analysis of peptidic analytes (2-10 kDa) in doping control urine samples'. Together they form a unique fingerprint.