Data from a microdosed recombinant human erythropoietin administration study applying the new biotinylated clone AE7A5 antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis protocol

Christian Reichel, Damir Erceg, Barbara Lorenc, Veronika Scheiblhofer, Letizia Farmer, Katharina Zanitzer, Thomas Geisendorfer, Günter Gmeiner, Mario Thevis

Publication: Contribution to journalJournal articlesResearchpeer-review

Abstract

Erythropoietin (EPO) is a hormone, which stimulates the production of red blood cells. Due to its performance-enhancing effect, it is prohibited by the World Anti-Doping Agency (WADA). In order to reduce the detection window of EPO doping, athletes have been applying low doses of recombinant EPO (e.g., <10 IU/kg body weight, daily or every second day) instead of larger doses twice or more per week (e.g., 30 IU/kg). Microdoses of Retacrit (epoetin zeta), an EPO biosimilar, were administered intravenously and subcutaneously to human males and females. Urine and serum samples were collected and analysed applying the new biotinylated clone AE7A5 EPO antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis (SAR-PAGE) protocol. With the improved protocol, microdosed Retacrit (7.5 IU/kg body weight [BW]) was detectable for at least 52 h after intravenous administration. Detection windows were approximately the same for serum and urine and doubled after subcutaneous administration (~104 h). Previous studies applying different electrophoretic techniques and the not further optimized SAR-PAGE protocol revealed considerably shorter detection windows for recombinant human erythropoietin (rhEPO) microdoses. Because the new biotinylated antibody performed significantly more sensitive than the nonbiotinylated version, the new protocol will improve the sensitivity and hence detectability of recombinant EPO in doping control.
Original languageEnglish
JournalDrug testing and analysis
Pages (from-to)1-10
Number of pages10
ISSN1942-7603
DOIs
Publication statusElectronically/ online published ahead of print - 15.01.2021

Research areas and keywords

  • doping control
  • epoetin zeta
  • erythropoietin
  • microdosis
  • SAR-PAGE

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