Detection method for recombinant human growth hormone based on 2D-PAGE

K. Püschel, Wilhelm Schänzer, Mario Thevis, M. Kohler, D. Sakharov, A. Tonevitskiy

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch


Human growth hormone (GH) has several central metabolic functions including bone growth in childhood and its anabolic and lipolytic effects in particular are assumed reasons for the abuse of GH by athletes. Human endogenous GH consists of a main 22 kDa variant and several isoforms. In contrast, recombinant GH consists of only one variant being identical to the main endogenous isoform. The method presented here separates different isoforms by 2D-PAGE after isolation of growth hormone from plasma using an immunoaffinity purification system. While samples containing endogenous GH yield up to four isoforms, samples with recombinant GH are supposed to contain the main 22 kDa spot only. External stimuli like exercise trigger the release of growth hormone from the pituitary. It was shown that the release is detected by the presented method as an increase in all isoforms. This approach detects isoforms of GH from plasma and discriminates endogenous GH from its recombinant analog which makes it a useful approach for doping control purposes. Verf.-Referat
Original languageGerman
Title of host publicationRecent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages11
PublisherSportverlag Strauß
Publication date2008
Publication statusPublished - 2008
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 24.02.200829.02.2008
Conference number: 26