Development and validation of a HPLC--QTOF-MS method for the determination of GHB-ß-O-glucuronide and GHB-4-sulfate in plasma and urine

Lena-Maria Mehling, Thomas Piper, Josef Dib, Daniel Sejer Pedersen, Burkhard Madea, Cornelius Hess, Mario Thevis

Publication: Contribution to journalJournal articlesResearchpeer-review

Abstract

Gamma-hydroxybutyric acid (GHB) is used as a so-called knock-out drug in drug-facilitated sexual assault. Due to GHB's short detection window in blood and urine, crime verification remains problematic in many cases. Phase II metabolites can enhance the detectability and prolong the detection window of some lower-molecular-mass molecules, as exemplified by ethanol. GHB-$-O-glucuronide (GHB-Gluc) was recently introduced as one phase II metabolite, and GHB-4-sulfate (GHB-Sulf) was also postulated to represent a urinary metabolite of exogenous GHB. To unambiguously verify the presence of GHB-Sulf in urine, the reference standard material of GHB-Sulf was synthesized, together with its deuterated analogue, GHB-Sulf-d 6. This reference standard material, together with GHB-Gluc and GHB-Gluc-d 4, was employed to develop and validate a quantitative method for determining both target analytes in plasma and urine samples. Compounds were separated by high-performance liquid chromatography on a Hypercarb column and detected by electrospray ionization time-of-flight-mass spectrometry. Calibration curves were linear over the entire calibration range up to 20 mg/L, and quantification limits of
Original languageEnglish
JournalForensic Toxicology
Volume35
Issue number1
Pages (from-to)77–85
Number of pages9
ISSN1860-8973
DOIs
Publication statusPublished - 2017

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