Identification of human pituitary growth hormone variants by mass spectrometry

Maxie Kohler, Andreas Thomas, Klaus Püschel, Wilhelm Schänzer, Mario Thevis

Publication: Contribution to journalJournal articlesResearch

Abstract

The heterogeneity of human endogenous growth hormone (GH) is used in doping control analysis to distinguish it from the homogeneous recombinant analogue in plasma samples. Pituitary GH variants were characterized by gel electrophoresis and mass spectrometry. Besides 22 and 20 kDa isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac 2 NeuAc modification presumably located at Thr 60.

Original languageEnglish
JournalJournal of proteome research
Volume8
Issue number2
Pages (from-to)1071-1076
Number of pages6
ISSN1535-3893
DOIs
Publication statusPublished - 01.02.2009

Research areas and keywords

  • Adult
  • Aged, 80 and over
  • Amino Acid Sequence
  • Doping in Sports
  • Female
  • Genetic Variation
  • Human Growth Hormone
  • Humans
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Molecular Sequence Data
  • Peptides
  • Pituitary Gland
  • Protein Isoforms
  • Young Adult

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