Mass spectrometry-based analysis of IGF-1 and hGH

Mario Thevis; Michael Bredehöft; Maxie Kohler; Wilhelm Schänzer

doi:10.1007/978-3-540-79088-4_9

Mass spectrometry-based analysis of IGF-1 and hGH

Mario Thevis, Michael Bredehöft, Maxie Kohler, Wilhelm Schänzer

Publication: Contribution to journal › Journal articles › Research

Abstract

Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.

Original language	English
Journal	Handbook of experimental pharmacology
Issue number	195
Pages (from-to)	201-207
Number of pages	7
ISSN	0171-2004
DOIs	https://doi.org/10.1007/978-3-540-79088-4_9
Publication status	Published - 01.01.2010

Research areas and keywords

Amino Acid Sequence
Doping in Sports
Electrophoresis, Gel, Two-Dimensional
Human Growth Hormone
Humans
Insulin-Like Growth Factor I
Mass Spectrometry
Molecular Sequence Data
Recombinant Proteins

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10.1007/978-3-540-79088-4_9

Citation

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title = "Mass spectrometry-based analysis of IGF-1 and hGH",

abstract = "Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.",

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doi = "10.1007/978-3-540-79088-4_9",

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AU - Thevis, Mario

AU - Bredehöft, Michael

AU - Kohler, Maxie

AU - Schänzer, Wilhelm

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.

AB - Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.

KW - Amino Acid Sequence

KW - Doping in Sports

KW - Electrophoresis, Gel, Two-Dimensional

KW - Human Growth Hormone

KW - Humans

KW - Insulin-Like Growth Factor I

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Recombinant Proteins

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DO - 10.1007/978-3-540-79088-4_9

M3 - Journal articles

C2 - 20020366

SN - 0171-2004

SP - 201

EP - 207

JO - Handbook of experimental pharmacology

JF - Handbook of experimental pharmacology

IS - 195

ER -

Mass spectrometry-based analysis of IGF-1 and hGH

Abstract

Research areas and keywords

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