New sample preparation procedure for the confirmation of stanozolol and its metabolites by means of GC/MSMS and LC/ESI-MSMS

Gregor Fußhöller, A. Koch, U. Mareck, Andreas Thomas, A. Schmechel, G. Sigmund, Mario Thevis, Wilhelm Schänzer

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch

Abstract

This paper describes a simple and fast sample preparation procedure allowing the simultaneous confirmation of stanozolol and its major urinary metabolites. The procedure consists of very efficient purification steps such as solid-phase extraction, several liquid-liquid extractions and acidic re-extraction followed by GC/MSMS and LC/ESI-MSMS detection, respectively. The sample preparation procedure allows the confirmation of stanozolol, 3’-OH-stanozolol, 4ß-OH-stanozolol and 16ß-OH-stanozolol in human urine. Based on SPE and LLE with subsequent re-extraction, this procedure makes it possible to generate highly purified extracts for GC/MSMS and LC/MSMS analysis, respectively. Excellent detection limits enable the long-term detection of stanozolol misuse in doping control. Particularly suitable for a prolonged traceability are the hydroxylated metabolites 4ß-OH-stanozolol and 16ß-OH-stanozolol. Aus dem Text
Original languageEnglish
Title of host publicationRecent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages10
PublisherSport & Buch Strauß
Publication date2006
Pages229-238
Publication statusPublished - 2006
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 04.06.200609.06.2006
Conference number: 24

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