Abstract
Bioactive peptides such as insulins, synthetic adrenocorticotrophic hormone (ACTH) analogue Synacthen, Gonadorelin (LHRH) and insulin-like growth factors (IGF) provide a reasonable potential for the misuse as performance enhancing agents and are prohibited in elite sports according to the list of banned substances established by the WADA. Currently, determination of these target analytes is possible by single assays only. The present method provides results for a preliminary approach to determine various prohibited peptides occurring in urine (e.g. Gonadorelin, Humalog (Insulin Lispro), Apidra (Insulin Glulisine), Novolog (Insulin Aspart), Lantus (Insulin Glargine), Porcine Insulin, Bovine Insulin, IGF-1 etc.) in one screening procedure. The method enables the effective, highly sensitive and specific screening for several different target analytes that are simultaneously purified and analysed by means of immunoaffinity purification, subsequent liquid-chromatographic separation and high resolution / high accuracy mass spectrometric determination. Principally, the approach is extendable to any banned peptide, if adequate antibodies are available. At the present status of the project only a limited number of analytes were implemented in the method. Verf.-Referat
| Original language | German |
|---|---|
| Title of host publication | Recent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010 |
| Editors | Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck |
| Number of pages | 9 |
| Publisher | Sportverlag Strauß |
| Publication date | 2010 |
| Pages | 109-117 |
| Publication status | Published - 2010 |
| Event | Cologne Workshop on Dope Analysis - Köln, Germany Duration: 07.03.2010 → 12.03.2010 Conference number: 28 |