Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells

Tim Koppen, Andreas Weckmann, Stefan Müller, Simon Staubach, Wilhelm Bloch, R Jürgen Dohmen, Tilo Schwientek

Publication: Contribution to journalJournal articlesResearch

Abstract

Distinct types of vesicles are formed in eukaryotic cells that conduct a variable set of functions depending on their origin. One subtype designated circulating microvesicles (MVs) provides a novel form of intercellular communication and recent work suggested the release and uptake of morphogens in vesicles by Drosophila cells. In this study, we have examined cells of the hemocyte-like cell lines Kc167 and S2 and identified secreted vesicles in the culture supernatant. The vesicles were isolated and found to have characteristics comparable to exosomes and plasma membrane MVs released by mammalian cells. In wingless-transfected cells, the full-length protein was detected in the vesicle isolates. Proteomics analyses of the vesicles identified 269 proteins that include various orthologs of marker proteins and proteins with putative functions in vesicle formation and release. Analogous to their mammalian counterparts, the subcellular origin of the vesicular constituents of both cell lines is dominated by membrane-associated and cytosolic proteins with functions that are consistent with their localization in MVs. The analyses revealed a significant overlap of the Kc167 and S2 vesicle proteomes and confirmed a close correlation with non-mammalian and mammalian exosomes.

Original languageEnglish
JournalProteomics
Volume11
Issue number22
Pages (from-to)4397-410
Number of pages14
DOIs
Publication statusPublished - 01.11.2011

Research areas and keywords

  • Animals
  • Blotting, Western
  • Cell Line
  • Centrifugation, Density Gradient
  • Drosophila
  • Exosomes
  • Insect Proteins
  • Proteome
  • Proteomics
  • Ubiquitin

Fingerprint

Dive into the research topics of 'Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells'. Together they form a unique fingerprint.

Citation