Qualitative determination of rapid acting insulin analogues in urine by means of LC-MS/MS

Andreas Thomas, Mario Thevis, Philippe Delahaut, Alain Bosseloir, Wilhelm Schänzer

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch

Abstract

After mass spectrometric characterisation and qualitative determination in human plasma of the synthetic insulin analogues were described in former studies, the aim of this project was the development of an analytical procedure to determine these substances in urine, which is the commonly collected doping control specimen. Renal excretion of intact human insulin as well as its rapid acting synthetic analogues are barely described in literature, and the expected concentrations ranged in low femtomole per mL levels, due to efficient adsorption and metabolic degradation in liver and kidney, so a mass spectrometric approach with highly sophisticated sample preparation procedure was required. An additional reason to alternate from plasma to urine insulin determination for the rapid acing analogues particularly, was the short half-life of insulins in blood after subcutaneous injection of these substances, so a prolonged detection time window after application was expected. A solid phase extraction (SPE) and immunoaffinity chromatography (IAC) sample preparation, followed by liquid chromatography tandem mass spectrometry was used for a fast and reliable qualitative determination of the target analytes in doping control samples. Aus dem Text (geändert)
Original languageEnglish
Title of host publicationRecent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages10
PublisherSport & Buch Strauß
Publication date2006
Pages367-376
Publication statusPublished - 2006
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 04.06.200609.06.2006
Conference number: 24

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