Screening for N-desmethyl- and N-bisdesmethyl metabolites of Sibutramine using liquid chromatography-tandem mass spectrometry

G. Sigmund, Mario Thevis, A.K. Schiffer, Wilhelm Schänzer

Publication: Chapter in Book/Report/Conference proceedingConference contribution - Article for conferenceResearch

Abstract

Since January 2006, the list of prohibited substances established by the World Anti-Doping Agency includes the antidepressant / anti-obesity drug Sibutramine. Due to its rapid degradation to its active metabolites N-desmethyl and N-bisdesmethyl Sibutramine, reference compounds were synthesized and included into an existing screening assay to allow the unambiguous determination of these metabolic products in human urine using liquid-liquid extraction followed by liquid chromatography – tandem mass spectrometry. Characteristic product ions, obtained after electrospray ionization and collision-induced dissociation, were elucidated using high resolution / high accuracy mass measurements with a hybrid linear ion trap / orbitrap mass analyzer. Einleitung
Original languageEnglish
Title of host publicationRecent advances in doping analysis (14) : Proceedings of the Manfred Donike Workshop ; 24th Cologne Workshop on Dope Analysis 4th to 9th June 2006
EditorsWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Number of pages4
PublisherSport & Buch Strauß
Publication date2006
Pages403-406
Publication statusPublished - 2006
EventCologne Workshop on Dope Analysis - Köln, Germany
Duration: 04.06.200609.06.2006
Conference number: 24

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