Stereoisomers in sports drug testing: Analytical strategies and applications

Publication: Contribution to journalJournal articlesResearchpeer-review

Abstract

Analytics employed in modern doping controls are designed to cover an extensive range of rather diverse classes of substances, all of which are banned in sport according to the list of prohibited substances and methods of doping, resulting from their potential to be performance-enhancing and/or harmful to health. Many of these bioactive substances or their metabolites are chiral, which are comprehensively characterized and, if appropriate analytical approaches are applied, can be clearly identified. In sports drug testing, the enantiomeric composition of relevant compounds is not considered in all instances, although differences of isomers concerning their biological activity have been established. To date, the separation of stereoisomers in doping controls is only applied for selected target compounds, but with the development of efficient chiral chromatographic stationary phases, the added value of information on e.g. racemic shifts during the metabolic biotransformation reactions of drugs has been recognized. The immense variability of the substance classes represents however a major challenge, especially because both 'classic' doping agents belonging to the category of lower molecular mass molecules (e.g. stimulants, β2-agonists, betablockers, corticoids, etc.) as well as larger molecules from the category of peptides and proteins necessitate consideration. In the present (mini)review, the current status of analytical techniques in the field of doping control analysis of stereoisomers is highlighted and critically reviewed.

Original languageEnglish
Article number463154
JournalJournal of chromatography. A
Volume1674
DOIs
Publication statusPublished - 05.07.2022

Research areas and keywords

  • Anabolic Agents/analysis
  • Central Nervous System Stimulants
  • Doping in Sports/methods
  • Performance-Enhancing Substances/analysis
  • Stereoisomerism
  • Substance Abuse Detection/methods

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