Updated protocols for the detection of Sotatercept and Luspatercept in human serum

Christian Reichel, Günter Gmeiner, Katja Walpurgis, Mario Thevis

Publication: Contribution to journalJournal articlesResearchpeer-review

Abstract

We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively high amount of antibody required per sample (10 μg) and the need of a secondary antibody for the final detection. The updated protocols overcome these limitations by antigen-antibody complex formation in solution followed by capture of the complex with anti-antibody-coated magnetic beads. They also omit the secondary antibody incubation step by usage of biotinylated primary antibodies, which can be directly incubated with streptavidin-HRP. Thus, the new protocols are faster, simpler, and cheaper and offer comparable sensitivities.

Original languageEnglish
JournalDrug testing and analysis
Volume10
Issue number11-12
Pages (from-to)1708-1713
Number of pages6
ISSN1942-7603
DOIs
Publication statusPublished - 11.09.2018

Research areas and keywords

  • Journal Article

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