13C/12C ratios of endogenous urinary steroids investigated for doping control purposes

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13C/12C ratios of endogenous urinary steroids investigated for doping control purposes. / Piper, Thomas ; Flenker, Ulrich; Mareck, Ute; Schänzer, Wilhelm.

in: Drug testing and analysis, Jahrgang 1, Nr. 2, 01.02.2009, S. 65-72.

Publikationen: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung

Bibtex

@article{eeb816c69da4482b9a3a3e50d30f6382,

title = "13C/12C ratios of endogenous urinary steroids investigated for doping control purposes",

abstract = "In order to detect the misuse of endogenous anabolic steroids such as testosterone by athletes a total of n = 1734 suspicious urine samples were investigated by gas chromatography/combustion/isotope ratio mass spectrometry throughout the years 2005, 2006 and 2007. The (13)C/(12)C ratio of a target substance (androsterone, a testosterone metabolite) was compared to the (13)C/(12)C ratio of an endogenous reference compound (11beta-hydroxyandrosterone).N = 1340 samples were investigated due to elevated testosterone/epitestosterone ratios, with n = 87 (6.5{\%}) exceptional findings regarding their isotopic ratios. An additional n = 164 samples were investigated because of elevated dehydroepiandrosterone concentrations, with n = 2 (1.2{\%}) exceptional findings. The remainder were subjected to isotope ratio analysis because of elevated androsterone levels or because this was requested by sports federations.Significant differences between female and male samples were found for the (13)C/(12)C ratios of androsterone and 11beta-hydroxyandrosterone but not for samples taken in or out of competition.A further n = 645 samples originating from other World Anti-Doping Agency accredited laboratories, mainly throughout Europe as well as South America, South Africa and Southeast Asia, were investigated. The (13)C/(12)C ratios of the urinary steroids differ significantly for each geographical region, reflecting the dietary status of the individuals.The system stability over time has been tested by repeated injections of a standard solution and repeated processing of frozen stored blank urine. Despite a drift over time in absolute (13)C/(12)C ratios, no significant change in the difference of (13)C/(12)C (11beta-hydroxyandrosterone) minus (13)C/(12)C (androsterone) could be observed.",

keywords = "Anabolic Agents, Androsterone, Carbon, Carbon Isotopes, Dehydroepiandrosterone, Doping in Sports, Epitestosterone, Female, Humans, Male, Mass Spectrometry, Substance Abuse Detection, Testosterone",

author = "Thomas Piper and Ulrich Flenker and Ute Mareck and Wilhelm Sch{\"a}nzer",

year = "2009",

month = "2",

day = "1",

doi = "10.1002/dta.15",

language = "English",

volume = "1",

pages = "65--72",

journal = "Drug testing and analysis",

issn = "1942-7603",

publisher = "John Wiley & Sons, Ltd",

number = "2",

}

RIS

TY - JOUR

T1 - 13C/12C ratios of endogenous urinary steroids investigated for doping control purposes

AU - Piper, Thomas

AU - Flenker, Ulrich

AU - Mareck, Ute

AU - Schänzer, Wilhelm

PY - 2009/2/1

Y1 - 2009/2/1

N2 - In order to detect the misuse of endogenous anabolic steroids such as testosterone by athletes a total of n = 1734 suspicious urine samples were investigated by gas chromatography/combustion/isotope ratio mass spectrometry throughout the years 2005, 2006 and 2007. The (13)C/(12)C ratio of a target substance (androsterone, a testosterone metabolite) was compared to the (13)C/(12)C ratio of an endogenous reference compound (11beta-hydroxyandrosterone).N = 1340 samples were investigated due to elevated testosterone/epitestosterone ratios, with n = 87 (6.5%) exceptional findings regarding their isotopic ratios. An additional n = 164 samples were investigated because of elevated dehydroepiandrosterone concentrations, with n = 2 (1.2%) exceptional findings. The remainder were subjected to isotope ratio analysis because of elevated androsterone levels or because this was requested by sports federations.Significant differences between female and male samples were found for the (13)C/(12)C ratios of androsterone and 11beta-hydroxyandrosterone but not for samples taken in or out of competition.A further n = 645 samples originating from other World Anti-Doping Agency accredited laboratories, mainly throughout Europe as well as South America, South Africa and Southeast Asia, were investigated. The (13)C/(12)C ratios of the urinary steroids differ significantly for each geographical region, reflecting the dietary status of the individuals.The system stability over time has been tested by repeated injections of a standard solution and repeated processing of frozen stored blank urine. Despite a drift over time in absolute (13)C/(12)C ratios, no significant change in the difference of (13)C/(12)C (11beta-hydroxyandrosterone) minus (13)C/(12)C (androsterone) could be observed.

AB - In order to detect the misuse of endogenous anabolic steroids such as testosterone by athletes a total of n = 1734 suspicious urine samples were investigated by gas chromatography/combustion/isotope ratio mass spectrometry throughout the years 2005, 2006 and 2007. The (13)C/(12)C ratio of a target substance (androsterone, a testosterone metabolite) was compared to the (13)C/(12)C ratio of an endogenous reference compound (11beta-hydroxyandrosterone).N = 1340 samples were investigated due to elevated testosterone/epitestosterone ratios, with n = 87 (6.5%) exceptional findings regarding their isotopic ratios. An additional n = 164 samples were investigated because of elevated dehydroepiandrosterone concentrations, with n = 2 (1.2%) exceptional findings. The remainder were subjected to isotope ratio analysis because of elevated androsterone levels or because this was requested by sports federations.Significant differences between female and male samples were found for the (13)C/(12)C ratios of androsterone and 11beta-hydroxyandrosterone but not for samples taken in or out of competition.A further n = 645 samples originating from other World Anti-Doping Agency accredited laboratories, mainly throughout Europe as well as South America, South Africa and Southeast Asia, were investigated. The (13)C/(12)C ratios of the urinary steroids differ significantly for each geographical region, reflecting the dietary status of the individuals.The system stability over time has been tested by repeated injections of a standard solution and repeated processing of frozen stored blank urine. Despite a drift over time in absolute (13)C/(12)C ratios, no significant change in the difference of (13)C/(12)C (11beta-hydroxyandrosterone) minus (13)C/(12)C (androsterone) could be observed.

KW - Anabolic Agents

KW - Androsterone

KW - Carbon

KW - Carbon Isotopes

KW - Dehydroepiandrosterone

KW - Doping in Sports

KW - Epitestosterone

KW - Female

KW - Humans

KW - Male

KW - Mass Spectrometry

KW - Substance Abuse Detection

KW - Testosterone

U2 - 10.1002/dta.15

DO - 10.1002/dta.15

M3 - Journal articles

C2 - 20355178

VL - 1

SP - 65

EP - 72

JO - Drug testing and analysis

JF - Drug testing and analysis

SN - 1942-7603

IS - 2

ER -

ID: 136341