1-DHEA identification in seized dietary supplement

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For several years, anabolic androgenic steroids have been offered on the “dietary supplement” market. Since 1996, mainly prohormones of testosterone and nandrolone have been available. Following their classification as schedule III controlled substances by the 2004 Anabolic Steroid Control Act, products appeared on the market containing steroids that were never marketed as approved drugs. In the present study, we report the detection of steroids in a product seized by the governmental body. The product “1-Androsterone” of the brand name “Advanced Muscle Science” was labelled to contain 100 mg of “1-Androstene-3b-ol,17-one” per capsule. The product was analyzed underivatized and as bis-TMS derivative by GC-MS. For comparison, 3β-hydroxy-5α-androst-1-en-17-one (1-DHEA) was prepared by reduction of 5α-androst-1-ene-3,17-dione with LS-Selectride. After soxhlet extraction of the capsule content with n-hexane, the steroid identity was additionally confirmed by nuclear magnetic resonance spectroscopy. The amount of 3β-hydroxy-5α-androst-1-en-17-one was determined with 115 mg/capsule. From the knowledge of generalsteroid metabolism and forced by the indication on the product label that “1-Androsterone“ converts to 1-Testosterone the expected urinary metabolites 3α-hydroxy-5α-androst-1-en-17-one, 5α-androst-1-ene-3α,17β-diol, and -3β,17β-diol were synthesized by reduction of 1-Testosterone or 5α-androst-1-ene-3,17-dione. Verf.-Referat
TitelRecent advances in doping analysis (18) : Proceedings of the Manfred-Donike-Workshop, 28th Cologne Workshop on Dope Analysis : 7th to 12th March 2010
Redakteure/-innenWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Herausgeber/inSportverlag Strauß
PublikationsstatusVeröffentlicht - 2010
VeranstaltungCologne Workshop on Dope Analysis - Köln, Deutschland
Dauer: 07.03.201012.03.2010
Konferenznummer: 28

ID: 186994

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