A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair

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A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair. / Grose, Richard; Hutter, Caroline; Bloch, Wilhelm; Thorey, Irmgard; Watt, Fiona M; Fässler, Reinhard; Brakebusch, Cord; Werner, Sabine.

in: Development (Cambridge, England), Jahrgang 129, Nr. 9, 01.05.2002, S. 2303-2315.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Harvard

Grose, R, Hutter, C, Bloch, W, Thorey, I, Watt, FM, Fässler, R, Brakebusch, C & Werner, S 2002, 'A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair', Development (Cambridge, England), Jg. 129, Nr. 9, S. 2303-2315.

APA

Grose, R., Hutter, C., Bloch, W., Thorey, I., Watt, F. M., Fässler, R., Brakebusch, C., & Werner, S. (2002). A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair. Development (Cambridge, England), 129(9), 2303-2315.

Vancouver

Bibtex

@article{92636fe43f304b0c859f01d00a61b2d2,
title = "A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair",
abstract = "Integrins are ubiquitous transmembrane receptors that play crucial roles in cell-cell and cell-matrix interactions. In this study, we have determined the effects of the loss of beta 1 integrins in keratinocytes in vitro and during cutaneous wound repair. Flow cytometry of cultured beta 1-deficient keratinocytes confirmed the absence of beta 1 integrins and showed downregulation of alpha 6 beta 4 but not of alpha v integrins. beta 1-null keratinocytes were characterised by poor adhesion to various substrates, by a reduced proliferation rate and by a strongly impaired migratory capacity. In vivo, the loss of beta 1 integrins in keratinocytes caused a severe defect in wound healing. beta 1-null keratinocytes showed impaired migration and were more densely packed in the hyperproliferative epithelium. Surprisingly, their proliferation rate was not reduced in early wounds and even increased in late wounds. The failure in re-epithelialisation resulted in a prolonged inflammatory response, leading to dramatic alterations in the expression of important wound-regulated genes. Ultimately, beta 1-deficient epidermis did cover the wound bed, but the epithelial architecture was abnormal. These findings demonstrate a crucial role of beta 1 integrins in keratinocyte migration and wound re-epithelialisation. Movies available on-line",
keywords = "Animals, Antigens, CD29, Cell Adhesion, Cell Communication, Cell Division, Cell Movement, Cells, Cultured, Gene Expression, Keratinocytes, Mice, Mice, Knockout, Models, Biological, Neovascularization, Physiologic, Skin, Wound Healing",
author = "Richard Grose and Caroline Hutter and Wilhelm Bloch and Irmgard Thorey and Watt, {Fiona M} and Reinhard F{\"a}ssler and Cord Brakebusch and Sabine Werner",
year = "2002",
month = may,
day = "1",
language = "English",
volume = "129",
pages = "2303--2315",
journal = "Development (Cambridge, England)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "9",

}

RIS

TY - JOUR

T1 - A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair

AU - Grose, Richard

AU - Hutter, Caroline

AU - Bloch, Wilhelm

AU - Thorey, Irmgard

AU - Watt, Fiona M

AU - Fässler, Reinhard

AU - Brakebusch, Cord

AU - Werner, Sabine

PY - 2002/5/1

Y1 - 2002/5/1

N2 - Integrins are ubiquitous transmembrane receptors that play crucial roles in cell-cell and cell-matrix interactions. In this study, we have determined the effects of the loss of beta 1 integrins in keratinocytes in vitro and during cutaneous wound repair. Flow cytometry of cultured beta 1-deficient keratinocytes confirmed the absence of beta 1 integrins and showed downregulation of alpha 6 beta 4 but not of alpha v integrins. beta 1-null keratinocytes were characterised by poor adhesion to various substrates, by a reduced proliferation rate and by a strongly impaired migratory capacity. In vivo, the loss of beta 1 integrins in keratinocytes caused a severe defect in wound healing. beta 1-null keratinocytes showed impaired migration and were more densely packed in the hyperproliferative epithelium. Surprisingly, their proliferation rate was not reduced in early wounds and even increased in late wounds. The failure in re-epithelialisation resulted in a prolonged inflammatory response, leading to dramatic alterations in the expression of important wound-regulated genes. Ultimately, beta 1-deficient epidermis did cover the wound bed, but the epithelial architecture was abnormal. These findings demonstrate a crucial role of beta 1 integrins in keratinocyte migration and wound re-epithelialisation. Movies available on-line

AB - Integrins are ubiquitous transmembrane receptors that play crucial roles in cell-cell and cell-matrix interactions. In this study, we have determined the effects of the loss of beta 1 integrins in keratinocytes in vitro and during cutaneous wound repair. Flow cytometry of cultured beta 1-deficient keratinocytes confirmed the absence of beta 1 integrins and showed downregulation of alpha 6 beta 4 but not of alpha v integrins. beta 1-null keratinocytes were characterised by poor adhesion to various substrates, by a reduced proliferation rate and by a strongly impaired migratory capacity. In vivo, the loss of beta 1 integrins in keratinocytes caused a severe defect in wound healing. beta 1-null keratinocytes showed impaired migration and were more densely packed in the hyperproliferative epithelium. Surprisingly, their proliferation rate was not reduced in early wounds and even increased in late wounds. The failure in re-epithelialisation resulted in a prolonged inflammatory response, leading to dramatic alterations in the expression of important wound-regulated genes. Ultimately, beta 1-deficient epidermis did cover the wound bed, but the epithelial architecture was abnormal. These findings demonstrate a crucial role of beta 1 integrins in keratinocyte migration and wound re-epithelialisation. Movies available on-line

KW - Animals

KW - Antigens, CD29

KW - Cell Adhesion

KW - Cell Communication

KW - Cell Division

KW - Cell Movement

KW - Cells, Cultured

KW - Gene Expression

KW - Keratinocytes

KW - Mice

KW - Mice, Knockout

KW - Models, Biological

KW - Neovascularization, Physiologic

KW - Skin

KW - Wound Healing

M3 - Journal articles

C2 - 11959837

VL - 129

SP - 2303

EP - 2315

JO - Development (Cambridge, England)

JF - Development (Cambridge, England)

SN - 0950-1991

IS - 9

ER -

ID: 72577