Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

AutorInnen

  • Laura Luosujärvi
  • Markus Haapala
  • Mario Thevis
  • Ville Saarela
  • Sami Franssila
  • Raimo A Ketola
  • Risto Kostiainen
  • Tapio Kotiaho

Forschungseinrichtungen

Details

A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-microAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations (M(+*)) and protonated molecules ([M + H](+)) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the M(+*) ions as precursor ions than using the [M + H](+) ions, and therefore the M(+*) ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2-20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R > or = 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%-9%. In addition to the GC-microAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-microAPCI-Orbitrap MS) was demonstrated.

OriginalspracheEnglisch
ZeitschriftJournal of the American Society for Mass Spectrometry
Jahrgang21
Heft2
Seiten (von - bis)310-316
Seitenumfang7
DOIs
PublikationsstatusVeröffentlicht - 01.02.2010

ID: 131240

DOI

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