Application of stable carbon isotope analysis to the detection of testosterone administration to cattle

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Standard

Application of stable carbon isotope analysis to the detection of testosterone administration to cattle. / Hebestreit, Moritz; Flenker, Ulrich; Buisson, Corinne; Andre, Francois; Le Bizec, Bruno; Fry, Hildburg; Lang, Melanie; Weigert, Angelika Preiss; Heinrich, Katharina; Hird, Simon; Schänzer, Wilhelm.

in: Journal of agricultural and food chemistry, Jahrgang 54, Nr. 8, 19.04.2006, S. 2850-2858.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Harvard

Hebestreit, M, Flenker, U, Buisson, C, Andre, F, Le Bizec, B, Fry, H, Lang, M, Weigert, AP, Heinrich, K, Hird, S & Schänzer, W 2006, 'Application of stable carbon isotope analysis to the detection of testosterone administration to cattle', Journal of agricultural and food chemistry, Jg. 54, Nr. 8, S. 2850-2858. https://doi.org/10.1021/jf0531353

APA

Hebestreit, M., Flenker, U., Buisson, C., Andre, F., Le Bizec, B., Fry, H., Lang, M., Weigert, A. P., Heinrich, K., Hird, S., & Schänzer, W. (2006). Application of stable carbon isotope analysis to the detection of testosterone administration to cattle. Journal of agricultural and food chemistry, 54(8), 2850-2858. https://doi.org/10.1021/jf0531353

Vancouver

Bibtex

@article{5993203f91594be9980ea4d2088fb536,
title = "Application of stable carbon isotope analysis to the detection of testosterone administration to cattle",
abstract = "The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.",
keywords = "Animals, Carbon Isotopes, Cattle, Chromatography, High Pressure Liquid, Female, Gas Chromatography-Mass Spectrometry, Legislation, Food, Male, Testosterone",
author = "Moritz Hebestreit and Ulrich Flenker and Corinne Buisson and Francois Andre and {Le Bizec}, Bruno and Hildburg Fry and Melanie Lang and Weigert, {Angelika Preiss} and Katharina Heinrich and Simon Hird and Wilhelm Sch{\"a}nzer",
year = "2006",
month = apr,
day = "19",
doi = "10.1021/jf0531353",
language = "English",
volume = "54",
pages = "2850--2858",
journal = "Journal of agricultural and food chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "8",

}

RIS

TY - JOUR

T1 - Application of stable carbon isotope analysis to the detection of testosterone administration to cattle

AU - Hebestreit, Moritz

AU - Flenker, Ulrich

AU - Buisson, Corinne

AU - Andre, Francois

AU - Le Bizec, Bruno

AU - Fry, Hildburg

AU - Lang, Melanie

AU - Weigert, Angelika Preiss

AU - Heinrich, Katharina

AU - Hird, Simon

AU - Schänzer, Wilhelm

PY - 2006/4/19

Y1 - 2006/4/19

N2 - The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.

AB - The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.

KW - Animals

KW - Carbon Isotopes

KW - Cattle

KW - Chromatography, High Pressure Liquid

KW - Female

KW - Gas Chromatography-Mass Spectrometry

KW - Legislation, Food

KW - Male

KW - Testosterone

U2 - 10.1021/jf0531353

DO - 10.1021/jf0531353

M3 - Journal articles

C2 - 16608200

VL - 54

SP - 2850

EP - 2858

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

SN - 0021-8561

IS - 8

ER -

ID: 136703