COMP in the Infrapatellar Fat Pad - Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study

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COMP in the Infrapatellar Fat Pad - Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study. / Grevenstein, David; Heilig, Juliane; Dargel, Jens et al.

in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Jahrgang 38, Nr. 4, 01.04.2020, S. 747-758.

Publikationen: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung › Begutachtung

Bibtex

@article{9dd675f4bd124994b4e0bd7d711ec398,

title = "COMP in the Infrapatellar Fat Pad - Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study",

abstract = "Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high-grade OA (mean age 63.8 ± 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 ± 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1β [IL-1β] and IL-6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix-metalloproteinase-3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. {\textcopyright} 2019 The Authors. Journal of Orthopaedic Research{\textregistered} published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society J Orthop Res 38:747-758, 2020.",

keywords = "Hoffa's fat pad, cartilage, infrapatellar fat pad, knee, osteoarthritis",

author = "David Grevenstein and Juliane Heilig and Jens Dargel and Johannes Oppermann and Peer Eysel and Christoph Brochhausen and Anja Niehoff",

note = "{\textcopyright} 2019 The Authors. Journal of Orthopaedic Research{\textregistered} published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. Online: 17.11.2019",

year = "2020",

month = apr,

day = "1",

doi = "10.1002/jor.24514",

language = "English",

volume = "38",

pages = "747--758",

journal = "Journal of orthopaedic research : official publication of the Orthopaedic Research Society",

issn = "1554-527X",

publisher = "John Wiley and Sons Inc.",

number = "4",

}

RIS

TY - JOUR

T1 - COMP in the Infrapatellar Fat Pad - Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study

AU - Grevenstein, David

AU - Heilig, Juliane

AU - Dargel, Jens

AU - Oppermann, Johannes

AU - Eysel, Peer

AU - Brochhausen, Christoph

AU - Niehoff, Anja

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high-grade OA (mean age 63.8 ± 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 ± 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1β [IL-1β] and IL-6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix-metalloproteinase-3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society J Orthop Res 38:747-758, 2020.

AB - Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high-grade OA (mean age 63.8 ± 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 ± 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1β [IL-1β] and IL-6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix-metalloproteinase-3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society J Orthop Res 38:747-758, 2020.

KW - Hoffa's fat pad

KW - cartilage

KW - infrapatellar fat pad

KW - knee

KW - osteoarthritis

UR - https://www.mendeley.com/catalogue/4700a300-ad1e-38a9-b4ec-b91d3887627b/

U2 - 10.1002/jor.24514

DO - 10.1002/jor.24514

M3 - Journal articles

C2 - 31696983

VL - 38

SP - 747

EP - 758

JO - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

SN - 1554-527X

SN - 0736-0266

IS - 4

ER -

ID: 5035297