Publikationen: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung
Detection of homologous blood transfusion. / Voß, Sven; Thevis, Mario; Schinkothe, T.; Schänzer, Wilhelm.
in: International journal of sports medicine, Jahrgang 28, 2007, S. 633-637.Publikationen: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung
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TY - JOUR
T1 - Detection of homologous blood transfusion
AU - Voß, Sven
AU - Thevis, Mario
AU - Schinkothe, T.
AU - Schänzer, Wilhelm
PY - 2007
Y1 - 2007
N2 - The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray® instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly indentify mixed red blood cell populations in homologous blood transfusion samples containing 0.3-0.2% of donor blood. Verf.-Referat
AB - The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray® instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly indentify mixed red blood cell populations in homologous blood transfusion samples containing 0.3-0.2% of donor blood. Verf.-Referat
KW - Antikörper
KW - Blutdoping
KW - Bluttransfusion
KW - Diagnostische Verfahren
KW - Doping
KW - Dopingkontrolle
KW - Hämatologie
KW - Sportmedizin
KW - Untersuchung, sportärztliche
KW - Untersuchungsmethode
KW - Zytologie
M3 - Journal articles
VL - 28
SP - 633
EP - 637
JO - International journal of sports medicine
JF - International journal of sports medicine
SN - 0172-4622
ER -
ID: 192920