Detection of homologous blood transfusion

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Detection of homologous blood transfusion. / Voß, Sven; Thevis, Mario; Schinkothe, T.; Schänzer, Wilhelm.

in: International journal of sports medicine, Jahrgang 28, 2007, S. 633-637.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

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@article{9bf49406b02f4396a59c7eb06f37cd08,
title = "Detection of homologous blood transfusion",
abstract = "The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray{\circledR} instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly indentify mixed red blood cell populations in homologous blood transfusion samples containing 0.3-0.2{\%} of donor blood. Verf.-Referat",
keywords = "Antik{\"o}rper, Blutdoping, Bluttransfusion, Diagnostische Verfahren, Doping, Dopingkontrolle, H{\"a}matologie, Sportmedizin, Untersuchung, sport{\"a}rztliche, Untersuchungsmethode, Zytologie",
author = "Sven Vo{\ss} and Mario Thevis and T. Schinkothe and Wilhelm Sch{\"a}nzer",
year = "2007",
language = "English",
volume = "28",
pages = "633--637",
journal = "International journal of sports medicine",
issn = "0172-4622",
publisher = "Georg Thieme Verlag",

}

RIS

TY - JOUR

T1 - Detection of homologous blood transfusion

AU - Voß, Sven

AU - Thevis, Mario

AU - Schinkothe, T.

AU - Schänzer, Wilhelm

PY - 2007

Y1 - 2007

N2 - The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray® instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly indentify mixed red blood cell populations in homologous blood transfusion samples containing 0.3-0.2% of donor blood. Verf.-Referat

AB - The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray® instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly indentify mixed red blood cell populations in homologous blood transfusion samples containing 0.3-0.2% of donor blood. Verf.-Referat

KW - Antikörper

KW - Blutdoping

KW - Bluttransfusion

KW - Diagnostische Verfahren

KW - Doping

KW - Dopingkontrolle

KW - Hämatologie

KW - Sportmedizin

KW - Untersuchung, sportärztliche

KW - Untersuchungsmethode

KW - Zytologie

M3 - Journal articles

VL - 28

SP - 633

EP - 637

JO - International journal of sports medicine

JF - International journal of sports medicine

SN - 0172-4622

ER -

ID: 192920