Development of two complementary LC–HRMS methods for analyzing sotatercept in dried blood spots for doping controls

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Abstract

Aim: sotatercept is a therapeutic Fc-fusion protein with erythropoiesis-stimulating activity. Due to a potential abuse of the drug by athletes in professional sports, a sensitive detection method is required. In sports drug testing, alternative matrices such as dried blood spots (DBS) are gaining increasing attention as they can provide several advantages over conventional matrices. Materials methods: Herein, two complementary LC–high-resolution mass spectrometry (HRMS) detection methods for sotatercept from DBS, an initial testing procedure (ITP) and a confirmation procedure (CP) were developed and validated for the first time. Both methods comprise an ultrasonication-assisted extraction, affinity enrichment, proteolytic digestion and HRMS detection. Results conclusion: For the multianalyte ITP, artificial samples fortified with sotatercept, luspatercept and bimagrumab, and authentic specimens containing bimagrumab were successfully analyzed as proof-of-concept. The validated detection methods for sotatercept are fit for purpose and the ITP was shown to be suitable for the detection of novel IgG-based pharmaceuticals in doping control DBS samples.
OriginalspracheEnglisch
ZeitschriftBioanalysis
Jahrgang11
Ausgabenummer10
Seiten (von - bis)923-940
Seitenumfang18
ISSN1757-6180
DOIs
PublikationsstatusVeröffentlicht - 20.06.2019

Fachgebiete und Schlagwörter

  • Activin receptor type II (ActRII)
  • LC-HRMS
  • affinity purification
  • doping
  • dried blood spots
  • proteolytic digestion
  • sport

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