Di(2-ethylhexyl) phthalate metabolites as markers for blood transfusion in doping control : intra-individual variability of urinary concentrations

Titel in Übersetzung: Di(2-ethylhexyl)phthalat-Metaboliten als Marker für Bluttransfusionen in der Dopinganalytik : intraindividuelle Variabilität von Urinkonzentrationen

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Abstract

To indicate homologous or autologous blood transfusion in sports drug testing, quantification of increased urinary concentrations of di(2-ethylhexyl) phthalate (DEHP) metabolites presents a promising approach; however, the possible intra-individual variation of the metabolite concentrations over time has not been well characterized. The aim of this study was to explore the intra-individual variability of urinary DEHP metabolites among seven volunteers without special occupational exposure to DEHP during one week (n = 253) in order to investigate the possibility of increased urinary concentrations of the metabolites caused by, for example, residential, dietary, or environmental exposure. Quantification of three DEHP metabolites – mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-oxohexyl) phthalate, and mono(2-ethyl-5-hydroxyhexyl) phthalate – was accomplished after enzymatic hydrolysis of urinary glucuronide conjugates and direct injection using isotope-dilution liquid chromatography-tandem mass spectrometry. Although urinary concentrations of DEHP metabolites showed considerable intra-individual variation, no increased values were observed comparable to the concentrations measured in urine specimens collected after blood transfusion. Verf.-Referat
Titel in ÜbersetzungDi(2-ethylhexyl)phthalat-Metaboliten als Marker für Bluttransfusionen in der Dopinganalytik : intraindividuelle Variabilität von Urinkonzentrationen
OriginalspracheEnglisch
ZeitschriftDrug testing and analysis
Jahrgang3
Seiten (von - bis)892-895
Seitenumfang4
ISSN1942-7603
PublikationsstatusVeröffentlicht - 2011

Fachgebiete und Schlagwörter

  • Biochemie
  • Bluttransfusion
  • Doping
  • Dopinganalyse
  • Dopingbekämpfung
  • Dopingkontrolle
  • Dopingnachweis
  • Flüssigkeitschromatographie
  • Markersubstanz
  • Massenspektrometrie
  • Metabolit
  • Untersuchung, empirische
  • Urinuntersuchung

Zitation