Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Standard

Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage. / Firner, Sara; Zaucke, Frank; Michael, Joern; Dargel, Jens; Schiwy-Bochat, Karl-Heinz; Heilig, Juliane; Rothschild, Markus Alexander; Eysel, Peer; Brüggemann, Gert-Peter; Niehoff, Anja.

in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Jahrgang 65, Nr. 10, 10.2017, S. 593-606.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Harvard

APA

Vancouver

Bibtex

@article{ebabad9d0d83449fa467776392c7a719,
title = "Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage",
abstract = "Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n=4) and OA (n=42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n=8; score 14.3 ± 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n=20; score 18.9 ± 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.",
keywords = "Aged, Aged, 80 and over, Cartilage, Articular, Case-Control Studies, Collagen Type II, Extracellular Matrix, Female, Humans, Knee Joint, Male, Middle Aged, Osteoarthritis, Protein Transport, Journal Article",
author = "Sara Firner and Frank Zaucke and Joern Michael and Jens Dargel and Karl-Heinz Schiwy-Bochat and Juliane Heilig and Rothschild, {Markus Alexander} and Peer Eysel and Gert-Peter Br{\"u}ggemann and Anja Niehoff",
year = "2017",
month = oct,
doi = "10.1369/0022155417729154",
language = "English",
volume = "65",
pages = "593--606",
journal = "The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "10",

}

RIS

TY - JOUR

T1 - Extracellular Distribution of Collagen II and Perifibrillar Adapter Proteins in Healthy and Osteoarthritic Human Knee Joint Cartilage

AU - Firner, Sara

AU - Zaucke, Frank

AU - Michael, Joern

AU - Dargel, Jens

AU - Schiwy-Bochat, Karl-Heinz

AU - Heilig, Juliane

AU - Rothschild, Markus Alexander

AU - Eysel, Peer

AU - Brüggemann, Gert-Peter

AU - Niehoff, Anja

PY - 2017/10

Y1 - 2017/10

N2 - Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n=4) and OA (n=42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n=8; score 14.3 ± 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n=20; score 18.9 ± 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.

AB - Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n=4) and OA (n=42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n=8; score 14.3 ± 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n=20; score 18.9 ± 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.

KW - Aged

KW - Aged, 80 and over

KW - Cartilage, Articular

KW - Case-Control Studies

KW - Collagen Type II

KW - Extracellular Matrix

KW - Female

KW - Humans

KW - Knee Joint

KW - Male

KW - Middle Aged

KW - Osteoarthritis

KW - Protein Transport

KW - Journal Article

U2 - 10.1369/0022155417729154

DO - 10.1369/0022155417729154

M3 - Journal articles

C2 - 28846474

VL - 65

SP - 593

EP - 606

JO - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

SN - 0022-1554

IS - 10

ER -

ID: 3123061