Genistein modulates the anti-tumor activity of cisplatin in MCF-7 breast and HT-29 colon cancer cells

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Genistein modulates the anti-tumor activity of cisplatin in MCF-7 breast and HT-29 colon cancer cells. / Hu, Xiao-Juan; Xie, Ming-Yong; Kluxen, Felix M; Diel, Patrick.

in: Archives of toxicology, Jahrgang 88, Nr. 3, 01.03.2014, S. 625-35.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

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@article{4b7c5754f03f4c459a0b0d0b5f1e1984,
title = "Genistein modulates the anti-tumor activity of cisplatin in MCF-7 breast and HT-29 colon cancer cells",
abstract = "The function of genistein (GEN) on tumor prevention and tumor promotion is discussed controversially. A possible interference of GEN with chemotherapy has been only rarely addressed so far. In this study, effects of GEN on the anti-tumor activity of cisplatin (CIS) were investigated in the presence and absence of estradiol (10(-10) M) in MCF-7 breast and HT-29 colon cancer cells. Cells were treated with graded concentrations of GEN (10(-4)-10(-6) M), E2, CIS and combinations. Cell growth, proliferation and apoptosis were determined as well as the expression level of PCNA, Ki67 and BCL-2 family members. CIS and GEN 10(-4) M inhibited cell growth and induced apoptosis in MCF-7 and HT-29 cells in the presence and absence of E2. Co-treatment with CIS and 10(-4)M GEN resulted in additive effects. In concentrations of 10(-5) and 10(-6) M, GEN stimulated cell growth in MCF-7 cells. It promoted proliferation, inhibited apoptosis and counteracted the anti-tumor activity of CIS in MCF-7 and HT-29 cells. Particularly the ability of CIS to induce apoptosis was antagonized. In ER alpha-positive MCF-7 cells, but not in ER alpha-negative HT-29 cells, E2 was able to neutralize the anti-CIS effects of GEN. Our data provide evidence that GEN in the absence of E2, a situation which occurs in postmenopausal women, directly affects the anti-tumor activity of cytostatic drugs like CIS. The exact molecular mechanism has to be investigated in future studies.",
keywords = "Antineoplastic Agents, Apoptosis, Cell Proliferation, Cisplatin, Drug Interactions, Estradiol, Estrogen Receptor alpha, Female, Genistein, HT29 Cells, Humans, MCF-7 Cells",
author = "Xiao-Juan Hu and Ming-Yong Xie and Kluxen, {Felix M} and Patrick Diel",
year = "2014",
month = mar,
day = "1",
doi = "10.1007/s00204-013-1184-4",
language = "English",
volume = "88",
pages = "625--35",
journal = "Archives of toxicology",
issn = "0340-5761",
publisher = "Springer Verlag",
number = "3",

}

RIS

TY - JOUR

T1 - Genistein modulates the anti-tumor activity of cisplatin in MCF-7 breast and HT-29 colon cancer cells

AU - Hu, Xiao-Juan

AU - Xie, Ming-Yong

AU - Kluxen, Felix M

AU - Diel, Patrick

PY - 2014/3/1

Y1 - 2014/3/1

N2 - The function of genistein (GEN) on tumor prevention and tumor promotion is discussed controversially. A possible interference of GEN with chemotherapy has been only rarely addressed so far. In this study, effects of GEN on the anti-tumor activity of cisplatin (CIS) were investigated in the presence and absence of estradiol (10(-10) M) in MCF-7 breast and HT-29 colon cancer cells. Cells were treated with graded concentrations of GEN (10(-4)-10(-6) M), E2, CIS and combinations. Cell growth, proliferation and apoptosis were determined as well as the expression level of PCNA, Ki67 and BCL-2 family members. CIS and GEN 10(-4) M inhibited cell growth and induced apoptosis in MCF-7 and HT-29 cells in the presence and absence of E2. Co-treatment with CIS and 10(-4)M GEN resulted in additive effects. In concentrations of 10(-5) and 10(-6) M, GEN stimulated cell growth in MCF-7 cells. It promoted proliferation, inhibited apoptosis and counteracted the anti-tumor activity of CIS in MCF-7 and HT-29 cells. Particularly the ability of CIS to induce apoptosis was antagonized. In ER alpha-positive MCF-7 cells, but not in ER alpha-negative HT-29 cells, E2 was able to neutralize the anti-CIS effects of GEN. Our data provide evidence that GEN in the absence of E2, a situation which occurs in postmenopausal women, directly affects the anti-tumor activity of cytostatic drugs like CIS. The exact molecular mechanism has to be investigated in future studies.

AB - The function of genistein (GEN) on tumor prevention and tumor promotion is discussed controversially. A possible interference of GEN with chemotherapy has been only rarely addressed so far. In this study, effects of GEN on the anti-tumor activity of cisplatin (CIS) were investigated in the presence and absence of estradiol (10(-10) M) in MCF-7 breast and HT-29 colon cancer cells. Cells were treated with graded concentrations of GEN (10(-4)-10(-6) M), E2, CIS and combinations. Cell growth, proliferation and apoptosis were determined as well as the expression level of PCNA, Ki67 and BCL-2 family members. CIS and GEN 10(-4) M inhibited cell growth and induced apoptosis in MCF-7 and HT-29 cells in the presence and absence of E2. Co-treatment with CIS and 10(-4)M GEN resulted in additive effects. In concentrations of 10(-5) and 10(-6) M, GEN stimulated cell growth in MCF-7 cells. It promoted proliferation, inhibited apoptosis and counteracted the anti-tumor activity of CIS in MCF-7 and HT-29 cells. Particularly the ability of CIS to induce apoptosis was antagonized. In ER alpha-positive MCF-7 cells, but not in ER alpha-negative HT-29 cells, E2 was able to neutralize the anti-CIS effects of GEN. Our data provide evidence that GEN in the absence of E2, a situation which occurs in postmenopausal women, directly affects the anti-tumor activity of cytostatic drugs like CIS. The exact molecular mechanism has to be investigated in future studies.

KW - Antineoplastic Agents

KW - Apoptosis

KW - Cell Proliferation

KW - Cisplatin

KW - Drug Interactions

KW - Estradiol

KW - Estrogen Receptor alpha

KW - Female

KW - Genistein

KW - HT29 Cells

KW - Humans

KW - MCF-7 Cells

U2 - 10.1007/s00204-013-1184-4

DO - 10.1007/s00204-013-1184-4

M3 - Journal articles

C2 - 24504162

VL - 88

SP - 625

EP - 635

JO - Archives of toxicology

JF - Archives of toxicology

SN - 0340-5761

IS - 3

ER -

ID: 739196