Identification of human pituitary growth hormone variants by mass spectrometry

Maxie Kohler, Andreas Thomas, Klaus Püschel, Wilhelm Schänzer, Mario Thevis

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Abstract

The heterogeneity of human endogenous growth hormone (GH) is used in doping control analysis to distinguish it from the homogeneous recombinant analogue in plasma samples. Pituitary GH variants were characterized by gel electrophoresis and mass spectrometry. Besides 22 and 20 kDa isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac 2 NeuAc modification presumably located at Thr 60.

OriginalspracheEnglisch
ZeitschriftJournal of proteome research
Jahrgang8
Ausgabenummer2
Seiten (von - bis)1071-1076
Seitenumfang6
ISSN1535-3893
DOIs
PublikationsstatusVeröffentlicht - 01.02.2009

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