Implementation and Performance of the Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry-Based Method for the Confirmatory Analysis of Endogenous Anabolic Steroids during the Rio de Janeiro Olympic and Paralympic Games 2016

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

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Implementation and Performance of the Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry-Based Method for the Confirmatory Analysis of Endogenous Anabolic Steroids during the Rio de Janeiro Olympic and Paralympic Games 2016. / de Oliveira, Fábio Azamor; Casilli, Alessandro; Piper, Thomas; da Silva, Thais Reis; da Silva, Cristiane Abrantes; da Silva, Raquel Vieira Santana; Dal Sasso, Marco Aurelio; Salgueiro, Gutierri Ricardo Dos Santos Gonçalves; Padilha, Monica Costa; Pereira, Henrique Marcelo Gualberto; Thevis, Mario; de Aquino Neto, Francisco Radler.

in: Analytical chemistry, Jahrgang 91, Nr. 18, 17.09.2019, S. 11747-11756.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Harvard

APA

de Oliveira, F. A., Casilli, A., Piper, T., da Silva, T. R., da Silva, C. A., da Silva, R. V. S., Dal Sasso, M. A., Salgueiro, G. R. D. S. G., Padilha, M. C., Pereira, H. M. G., Thevis, M., & de Aquino Neto, F. R. (2019). Implementation and Performance of the Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry-Based Method for the Confirmatory Analysis of Endogenous Anabolic Steroids during the Rio de Janeiro Olympic and Paralympic Games 2016. Analytical chemistry, 91(18), 11747-11756. https://doi.org/10.1021/acs.analchem.9b02341

Vancouver

Bibtex

@article{a96dae9c80404fa6ae548797ffb17574,
title = "Implementation and Performance of the Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry-Based Method for the Confirmatory Analysis of Endogenous Anabolic Steroids during the Rio de Janeiro Olympic and Paralympic Games 2016",
abstract = "Carbon isotope ratio (CIR) confirmation is one of the most complex and delicate analyses in the doping control field, due to the nature of the molecules to be confirmed, normally present in urinary samples as a consequence of an endogenous production. The requirements for method validation established by the World Anti-Doping Agency (WADA) have been pushing the accredited laboratories to improve their methods. The choice of the method is always a cost benefit ratio involving a hard-working and time-consuming analysis and the guarantee of reporting of reliable results. This work presents the method fully validated by the Brazilian Doping Control Laboratory as part of the preparation for the Rio de Janeiro Summer Olympic and Paralympic Games 2016. Sample preparation encompassed solid-phase extraction, liquid-liquid extraction, enzymatic hydrolysis, acetylation, and purification by preparative high-performance liquid chromatography, and analyses were performed by gas chromatography/combustion/isotope ratio mass spectrometry. This proved to be a robust method to CIR confirmation in a big event, as demonstrated by the analysis of 179 samples during the Games 2016, from clearly negative results and adverse findings for testosterone (T) and related substances, boldenone and its metabolite, 19-norandrosterone and formestane. Two atypical findings were also reported for T and metabolites.",
author = "{de Oliveira}, {F{\'a}bio Azamor} and Alessandro Casilli and Thomas Piper and {da Silva}, {Thais Reis} and {da Silva}, {Cristiane Abrantes} and {da Silva}, {Raquel Vieira Santana} and {Dal Sasso}, {Marco Aurelio} and Salgueiro, {Gutierri Ricardo Dos Santos Gon{\c c}alves} and Padilha, {Monica Costa} and Pereira, {Henrique Marcelo Gualberto} and Mario Thevis and {de Aquino Neto}, {Francisco Radler}",
note = "Online 19.08.2019",
year = "2019",
month = sep,
day = "17",
doi = "10.1021/acs.analchem.9b02341",
language = "English",
volume = "91",
pages = "11747--11756",
journal = "Analytical chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "18",

}

RIS

TY - JOUR

T1 - Implementation and Performance of the Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry-Based Method for the Confirmatory Analysis of Endogenous Anabolic Steroids during the Rio de Janeiro Olympic and Paralympic Games 2016

AU - de Oliveira, Fábio Azamor

AU - Casilli, Alessandro

AU - Piper, Thomas

AU - da Silva, Thais Reis

AU - da Silva, Cristiane Abrantes

AU - da Silva, Raquel Vieira Santana

AU - Dal Sasso, Marco Aurelio

AU - Salgueiro, Gutierri Ricardo Dos Santos Gonçalves

AU - Padilha, Monica Costa

AU - Pereira, Henrique Marcelo Gualberto

AU - Thevis, Mario

AU - de Aquino Neto, Francisco Radler

N1 - Online 19.08.2019

PY - 2019/9/17

Y1 - 2019/9/17

N2 - Carbon isotope ratio (CIR) confirmation is one of the most complex and delicate analyses in the doping control field, due to the nature of the molecules to be confirmed, normally present in urinary samples as a consequence of an endogenous production. The requirements for method validation established by the World Anti-Doping Agency (WADA) have been pushing the accredited laboratories to improve their methods. The choice of the method is always a cost benefit ratio involving a hard-working and time-consuming analysis and the guarantee of reporting of reliable results. This work presents the method fully validated by the Brazilian Doping Control Laboratory as part of the preparation for the Rio de Janeiro Summer Olympic and Paralympic Games 2016. Sample preparation encompassed solid-phase extraction, liquid-liquid extraction, enzymatic hydrolysis, acetylation, and purification by preparative high-performance liquid chromatography, and analyses were performed by gas chromatography/combustion/isotope ratio mass spectrometry. This proved to be a robust method to CIR confirmation in a big event, as demonstrated by the analysis of 179 samples during the Games 2016, from clearly negative results and adverse findings for testosterone (T) and related substances, boldenone and its metabolite, 19-norandrosterone and formestane. Two atypical findings were also reported for T and metabolites.

AB - Carbon isotope ratio (CIR) confirmation is one of the most complex and delicate analyses in the doping control field, due to the nature of the molecules to be confirmed, normally present in urinary samples as a consequence of an endogenous production. The requirements for method validation established by the World Anti-Doping Agency (WADA) have been pushing the accredited laboratories to improve their methods. The choice of the method is always a cost benefit ratio involving a hard-working and time-consuming analysis and the guarantee of reporting of reliable results. This work presents the method fully validated by the Brazilian Doping Control Laboratory as part of the preparation for the Rio de Janeiro Summer Olympic and Paralympic Games 2016. Sample preparation encompassed solid-phase extraction, liquid-liquid extraction, enzymatic hydrolysis, acetylation, and purification by preparative high-performance liquid chromatography, and analyses were performed by gas chromatography/combustion/isotope ratio mass spectrometry. This proved to be a robust method to CIR confirmation in a big event, as demonstrated by the analysis of 179 samples during the Games 2016, from clearly negative results and adverse findings for testosterone (T) and related substances, boldenone and its metabolite, 19-norandrosterone and formestane. Two atypical findings were also reported for T and metabolites.

UR - https://www.mendeley.com/catalogue/fffb83b7-1f94-35cc-bd0d-e012f952fe43/

U2 - 10.1021/acs.analchem.9b02341

DO - 10.1021/acs.analchem.9b02341

M3 - Journal articles

C2 - 31423767

VL - 91

SP - 11747

EP - 11756

JO - Analytical chemistry

JF - Analytical chemistry

SN - 0003-2700

IS - 18

ER -

ID: 4713038