Abstract
Mass spectrometry, in combination with gas or liquid chromatography (GCMS or LCMS), is the analytical technique of choice for drug testing in sport. To identify the presence of anabolic steroids at trace levels, tandem mass spectrometry (MS/MS) has been applied to gas chromatography based analytical methods. In case of analyzing samples with complex matrices, the application of ion traps or triple quadrupole instruments offers an increased sensitivity und selectivity by an effective elimination of the matrix background. Ion traps are characterized by their high sensitivity in full scan and product ion scan mode and offer the possibility for MSn. However, due to long cycle times the number of analytes that can be determined simultaneously in MS/MS mode is restricted to 4-5. Furthermore, the quantitation capability and the ion ratio stability are poor. In contrast, triple quadrupole instruments are known for their high sensitivity, selectivity, ion ratio stability and linearity. Thus accurate and reproducible quantification is possible - even in the presence of complex matrices. Additionally, in selected reaction monitoring mode (SRM) these instruments provide the opportunity to test 25-30 analytes simultaneously. The objective of this work has been to compare the new TSQ Quantum GC triple quadrupole instrument and the PolarisQ ion trap system when analyzing anabolic steroids in human urine. The comparison of these two MS/MS techniques is carried out by considering the parameters linearity, ion ratio stability and reproducibility. The sensitivity of the new GC triple quadrupole instrument is shown by means of selected target analytes. Einleitung
Originalsprache | Englisch |
---|---|
Titel | Recent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008 |
Herausgeber*innen | Wilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck |
Seitenumfang | 4 |
Herausgeber (Verlag) | Sportverlag Strauß |
Erscheinungsdatum | 2008 |
Seiten | 285-288 |
Publikationsstatus | Veröffentlicht - 2008 |
Veranstaltung | Cologne Workshop on Dope Analysis - Köln, Deutschland Dauer: 24.02.2008 → 29.02.2008 Konferenznummer: 26 |
Fachgebiete und Schlagwörter
- Biochemie
- Doping
- Dopingnachweis
- Gaschromatographie
- Massenspektrometrie
- Messverfahren
- Screening
- Sportmedizin
- Steroid, anaboles
- Urinuntersuchung