Improved screening of anabolic steroids in human urine using a new GC-triple quadrupole mass spectrometer : preliminary results

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitrag - Aufsatz in KonferenzbandForschung

Abstract

Mass spectrometry, in combination with gas or liquid chromatography (GCMS or LCMS), is the analytical technique of choice for drug testing in sport. To identify the presence of anabolic steroids at trace levels, tandem mass spectrometry (MS/MS) has been applied to gas chromatography based analytical methods. In case of analyzing samples with complex matrices, the application of ion traps or triple quadrupole instruments offers an increased sensitivity und selectivity by an effective elimination of the matrix background. Ion traps are characterized by their high sensitivity in full scan and product ion scan mode and offer the possibility for MSn. However, due to long cycle times the number of analytes that can be determined simultaneously in MS/MS mode is restricted to 4-5. Furthermore, the quantitation capability and the ion ratio stability are poor. In contrast, triple quadrupole instruments are known for their high sensitivity, selectivity, ion ratio stability and linearity. Thus accurate and reproducible quantification is possible - even in the presence of complex matrices. Additionally, in selected reaction monitoring mode (SRM) these instruments provide the opportunity to test 25-30 analytes simultaneously. The objective of this work has been to compare the new TSQ Quantum GC triple quadrupole instrument and the PolarisQ ion trap system when analyzing anabolic steroids in human urine. The comparison of these two MS/MS techniques is carried out by considering the parameters linearity, ion ratio stability and reproducibility. The sensitivity of the new GC triple quadrupole instrument is shown by means of selected target analytes. Einleitung
OriginalspracheEnglisch
TitelRecent advances in doping analysis (16) : Proceedings of the Manfred Donike Workshop ; 26th Cologne Workshop on Dope Analysis, 24th to 29th February 2008
Herausgeber*innenWilhelm Schänzer, Hans Geyer, A. Gotzmann, Ute Mareck
Seitenumfang4
Herausgeber (Verlag)Sportverlag Strauß
Erscheinungsdatum2008
Seiten285-288
PublikationsstatusVeröffentlicht - 2008
VeranstaltungCologne Workshop on Dope Analysis - Köln, Deutschland
Dauer: 24.02.200829.02.2008
Konferenznummer: 26

Fachgebiete und Schlagwörter

  • Biochemie
  • Doping
  • Dopingnachweis
  • Gaschromatographie
  • Massenspektrometrie
  • Messverfahren
  • Screening
  • Sportmedizin
  • Steroid, anaboles
  • Urinuntersuchung

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