Influence of endostatin on embryonic vasculo- and angiogenesis

Annette Schmidt, Daniela Wenzel, Isabell Ferring, Shohreh Kazemi, Takako Sasaki, Jürgen Hescheler, Rupert Timpl, Klaus Addicks, Bernd K Fleischmann, Wilhelm Bloch

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

Abstract

The proteolytic fragment of collagen XVIII, endostatin, acts as an inhibitor of angiogenesis. To date, only limited knowledge exists on the effects of endostatin on endothelial cells during embryonic development. Therefore, we analysed the role of endostatin on embryonic vasculo- and angiogenesis. Endostatin is accumulated in embryonic tissue of mouse embryos. Similarly, in vessels of embryoid bodies (EBs), endostatin and its binding sites are distributed in vessels and sprouting areas. In EBs, endostatin increases endothelial cells (control, 279.3 +/- 76.5; 50 ng/ml, 566.3 +/- 90.1; 200 ng/ml, 594.5 +/- 166.3 tube-like structures per EB) and endothelial tubes by proliferation (control, 21.4 +/- 7.5; 50 ng/ml, 160.2 +/- 9.9; 200 ng/ml, 184.2 +/- 16.5 Ki67-positive nuclei per 50 tube-like structures); it also enhances migration (control, 380.5 +/- 159.8 cells; 50 ng/ml, 718.3 +/- 251 cells; 200 ng/ml, 706 +/- 89.4 cells) and apoptosis (control, 16.8 +/- 6.7; 50 ng/ml, 94.4 +/- 23.6; 200 ng/ml, 106 +/- 42 PARP-positive nuclei per 50 tube-like structures). Furthermore, endostatin modulates the morphology of the endothelial tubes by inducing contraction. Endostatin modulates the embryonic vascular development by enhancing proliferation, migration, and apoptosis as well as by regulating morphogenesis.

OriginalspracheEnglisch
ZeitschriftDevelopmental dynamics : an official publication of the American Association of Anatomists
Jahrgang230
Ausgabenummer3
Seiten (von - bis)468-480
Seitenumfang13
ISSN1058-8388
DOIs
PublikationsstatusVeröffentlicht - 01.07.2004

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