Oxidative and nitrosative stress and apoptosis in oral mucosa cells after ex vivo exposure to lead and benzo[a]pyrene

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Standard

Oxidative and nitrosative stress and apoptosis in oral mucosa cells after ex vivo exposure to lead and benzo[a]pyrene. / Wannhoff, A; Bölck, B; Kübler, A C; Bloch, W; Reuther, T.

in: Toxicology in vitro : an international journal published in association with BIBRA, Jahrgang 27, Nr. 2, 01.03.2013, S. 915-21.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Harvard

APA

Vancouver

Bibtex

@article{7a72364e5563489283ccb81f956345e2,
title = "Oxidative and nitrosative stress and apoptosis in oral mucosa cells after ex vivo exposure to lead and benzo[a]pyrene",
abstract = "Exposure of human oral mucosa to lead (Pb) and benzo[a]pyrene (BaP) by inhalation and ingestion can lead to pathological conditions via apoptosis and oxidative and nitrosative stress. However, few studies have investigated the effects of Pb and BaP on oral mucosa cells. Furthermore, previous studies focused on chronic Pb and BaP exposure. Therefore, we evaluated important markers of apoptosis and oxidative and nitrosative stress in oral mucosa cells by incubating the cells with Pb and BaP for 5-360 min. Ex vivo samples of human oral mucosa were exposed to Pb or BaP, and immunohistochemical staining was performed to evaluate active caspase-3, 8-epi-prostaglandin F2 alpha (8-epi-PGF2a), and 3-nitrotyrosine (3-NT). Pb and BaP treatments significantly increased active caspase-3 levels in a time-dependent manner. Furthermore, the treatments induced an early increase in 3-NT level, which ceased with longer incubation times. 8-Epi-PGF2a level increased only after prolonged incubation with Pb, and this elevation was irrespective of BaP incubation duration. Smokers' samples had significantly lower levels of markers of oxidative and nitrosative stress than did nonsmokers' samples. Thus, single, short-term exposure to Pb or BaP increases the levels of apoptosis markers and markers of oxidative and nitrosative stress.",
keywords = "Adolescent, Adult, Apoptosis, Benzo(a)pyrene, Caspase 3, Dinoprost, Environmental Pollutants, Female, Humans, Lead, Male, Middle Aged, Mouth Mucosa, Oxidative Stress, Smoking, Tyrosine, Young Adult",
author = "A Wannhoff and B B{\"o}lck and K{\"u}bler, {A C} and W Bloch and T Reuther",
note = "Copyright {\textcopyright} 2013 Elsevier Ltd. All rights reserved.",
year = "2013",
month = mar,
day = "1",
doi = "10.1016/j.tiv.2013.01.007",
language = "English",
volume = "27",
pages = "915--21",
journal = "Toxicology in vitro : an international journal published in association with BIBRA",
issn = "1879-3177",
publisher = "Elsevier Limited",
number = "2",

}

RIS

TY - JOUR

T1 - Oxidative and nitrosative stress and apoptosis in oral mucosa cells after ex vivo exposure to lead and benzo[a]pyrene

AU - Wannhoff, A

AU - Bölck, B

AU - Kübler, A C

AU - Bloch, W

AU - Reuther, T

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - Exposure of human oral mucosa to lead (Pb) and benzo[a]pyrene (BaP) by inhalation and ingestion can lead to pathological conditions via apoptosis and oxidative and nitrosative stress. However, few studies have investigated the effects of Pb and BaP on oral mucosa cells. Furthermore, previous studies focused on chronic Pb and BaP exposure. Therefore, we evaluated important markers of apoptosis and oxidative and nitrosative stress in oral mucosa cells by incubating the cells with Pb and BaP for 5-360 min. Ex vivo samples of human oral mucosa were exposed to Pb or BaP, and immunohistochemical staining was performed to evaluate active caspase-3, 8-epi-prostaglandin F2 alpha (8-epi-PGF2a), and 3-nitrotyrosine (3-NT). Pb and BaP treatments significantly increased active caspase-3 levels in a time-dependent manner. Furthermore, the treatments induced an early increase in 3-NT level, which ceased with longer incubation times. 8-Epi-PGF2a level increased only after prolonged incubation with Pb, and this elevation was irrespective of BaP incubation duration. Smokers' samples had significantly lower levels of markers of oxidative and nitrosative stress than did nonsmokers' samples. Thus, single, short-term exposure to Pb or BaP increases the levels of apoptosis markers and markers of oxidative and nitrosative stress.

AB - Exposure of human oral mucosa to lead (Pb) and benzo[a]pyrene (BaP) by inhalation and ingestion can lead to pathological conditions via apoptosis and oxidative and nitrosative stress. However, few studies have investigated the effects of Pb and BaP on oral mucosa cells. Furthermore, previous studies focused on chronic Pb and BaP exposure. Therefore, we evaluated important markers of apoptosis and oxidative and nitrosative stress in oral mucosa cells by incubating the cells with Pb and BaP for 5-360 min. Ex vivo samples of human oral mucosa were exposed to Pb or BaP, and immunohistochemical staining was performed to evaluate active caspase-3, 8-epi-prostaglandin F2 alpha (8-epi-PGF2a), and 3-nitrotyrosine (3-NT). Pb and BaP treatments significantly increased active caspase-3 levels in a time-dependent manner. Furthermore, the treatments induced an early increase in 3-NT level, which ceased with longer incubation times. 8-Epi-PGF2a level increased only after prolonged incubation with Pb, and this elevation was irrespective of BaP incubation duration. Smokers' samples had significantly lower levels of markers of oxidative and nitrosative stress than did nonsmokers' samples. Thus, single, short-term exposure to Pb or BaP increases the levels of apoptosis markers and markers of oxidative and nitrosative stress.

KW - Adolescent

KW - Adult

KW - Apoptosis

KW - Benzo(a)pyrene

KW - Caspase 3

KW - Dinoprost

KW - Environmental Pollutants

KW - Female

KW - Humans

KW - Lead

KW - Male

KW - Middle Aged

KW - Mouth Mucosa

KW - Oxidative Stress

KW - Smoking

KW - Tyrosine

KW - Young Adult

U2 - 10.1016/j.tiv.2013.01.007

DO - 10.1016/j.tiv.2013.01.007

M3 - Journal articles

C2 - 23318731

VL - 27

SP - 915

EP - 921

JO - Toxicology in vitro : an international journal published in association with BIBRA

JF - Toxicology in vitro : an international journal published in association with BIBRA

SN - 1879-3177

SN - 0887-2333

IS - 2

ER -

ID: 254502