Reductive gas-phase chemiluminescence and flow injection analysis for measurement of the nitric oxide pool in biological matrices

Ulrike Hendgen-Cotta, Marijke Grau, Tienush Rassaf, Putrika Gharini, Malte Kelm, Petra Kleinbongard

Publikation: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Abstract

There is growing evidence for nitric oxide (NO.) being involved in cell signaling and pathology. Much effort has been made to elucidate and characterize the different biochemical reaction pathways of NO.in vivo. However, a major obstacle in assessing the significance of nitrosated species and oxidized metabolites often remains: a reliable analytical technique for the detection of NO. in complex biological matrices. This chapter presents refined methodologies, such as chemiluminescence detection and flow injection analysis, compared with adequate sample processing procedures to reliably quantify and assess the circulating and resident NO(.) pool, consisting of nitrite, nitrate, nitroso, and nitrosylated species.

OriginalspracheEnglisch
ZeitschriftMethods in enzymology
Jahrgang441
Seiten (von - bis)295-315
Seitenumfang21
ISSN0076-6879
DOIs
PublikationsstatusVeröffentlicht - 01.01.2008

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