Response of serum concentration of cartilage biomarkers to 21-days of bed rest with exercise and nutrition countermeasures

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Introduction: Altered mechanical loading acting on the musculoskeletal system, like in space, can cause a disruption of articular cartilage homeostasis leading to degeneration. To date, the impact of reduced mechanical loading on articular cartilage is not well understood. Bed rest studies offer a unique model to study not only the effect of immobilization for several weeks but also of countermeasures on cartilage health in a well standardized environment. Cartilage oligomeric matrix protein (COMP), matrix metalloproteinases-3 (MMP-3) and chitinase-3-like 1 (YKL-40) measured in the blood serum are well-established biomarkers reflecting joint metabolism (Kraus et al., 2010). The aim of this study was to investigate the effect of a resistive vibration exercise protocol with and without nutritional supplements as countermeasures over 21-days of 6°-HDT (Head Down Tilt) bed rest on the serum concentration of articular cartilage biomarkers.

Methods: The “Medium duration nutrition and vibration exercise” (MNX) study was executed at the Institute for Space Medicine and Physiology (MEDES Clinique d’Investigation, Toulouse, France) under the direction of the French (CNES) and European Space Agency (ESA) in 2012 and 2013. The study was conducted in a cross-over design with three different campaigns each lasting 35 days. Each campaign consisted of 7 days of basic data collection (BDC-7 – BDC-1), 21 days 6°-HDT bed rest + countermeasure (HDT1 - HDT21) and a 7 day recovery period (R+1 – R+7). The three campaigns were separated by 4 months to prevent carry-over effects. The following countermeasures were implemented during the 21-days of bed rest: resistive vibration exercise (RVE), nutrition + resistive vibration exercise (NeX) and a control condition (CON). Each participant completed all three study campaigns with a randomized order. Blood samples were drawn on eleven days (BDC-3, BDC-2, BDC-1, HDT2, HDT3, HDT5, HDT7, HDT14, HDT21, R+1 and R+6). Serum COMP, MMP-3 and YKL-40 concentrations were analyzed using commercial enzyme-linked immunosorbent assays (ELISA) (COMP: AnaMar® Medical AB, Lund, Sweden; MMP-3: Quantikine®, Bio-Techne, R&D Systems, Minneapolis, USA; YKL-40: MicroVueTM® Bone, Quidel, San Diego, USA). A two-way (time and group) analysis of variance (ANOVA) with repeated measures was used to detect differences (p
Results/Discussion: Serum COMP concentration (N=8) decreased significantly on the first day of bed rest (p
References: Kraus et al. (2010). Osteoarthritis Cartilage 19:515-542.
Funding: Federal Ministry of Economic Affairs and Energy, Germany (DLR 50WB1217, DLR 50WB1520, DLR 50WB2021, DLR 50WB2022)
Titel6th Human Physiology Workshop
Herausgeber (Verlag)Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
PublikationsstatusVeröffentlicht - 04.12.2022
VeranstaltungHuman Physiology Workshop - virtual
Dauer: 04.12.202104.12.2021
Konferenznummer: 6

ID: 7376296

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