Serotonin Shapes the Migratory Potential of NK Cells: An in vitro Approach

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Serotonin Shapes the Migratory Potential of NK Cells : An in vitro Approach. / Zimmer, Philipp; Bloch, Wilhelm; Kieven, Markus; Lövenich, Lukas; Lehmann, Jonas; Holthaus, Michelle; Theurich, Sebastian; Schenk, Alexander.

in: International journal of sports medicine, Jahrgang 38, Nr. 11, 10.2017, S. 857-863.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

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@article{91a52ab817ed49bfa4c801b5e2616802,
title = "Serotonin Shapes the Migratory Potential of NK Cells: An in vitro Approach",
abstract = "Increased serotonin (5-HT) levels have been shown to influence natural killer cell (NK cell) function. Acute exercise mobilizes and activates NK cells and further increases serum 5-HT concentrations in a dose-dependent manner. The aim of this study was to investigate the impact of different serum 5-HT concentrations on NK cell migratory potential and cytotoxicity. The human NK cell line KHYG-1 was assigned to 4 conditions, including 3 physiological concentrations of 5-HT (100, 130 or 170 µg/l 5-HT) and one control condition. NK cells were analyzed regarding cytotoxicity, migratory potential and expression of adhesion molecules. No treatment effect on NK cell cytotoxicity and expression of integrin subunits was detected. Migratory potential was increased in a dose dependent manner, indicating the highest protease activity in cells that were incubated with 170 µg/l 5-HT (170 µg/l vs. control, p<0.001, 170 µg/l vs. 100 µg/l, p<0.001; 170 µg/l vs. 130 µg/l, p=0.003; 130 µg/l vs. control, p<0.001, 130 µg/l vs. 100 µg/l, p<0.001). These results suggest that elevated 5-HT serum levels play a mediating role in NK cell function. As exercise has been shown to be involved in NK cell mobilization and redistribution, the influence of 5-HT should be investigated in ex vivo and in vivo experiments.",
keywords = "Cell Line, Cell Movement, Exercise, Humans, Integrins, Killer Cells, Natural, Serotonin, Journal Article",
author = "Philipp Zimmer and Wilhelm Bloch and Markus Kieven and Lukas L{\"o}venich and Jonas Lehmann and Michelle Holthaus and Sebastian Theurich and Alexander Schenk",
note = "{\textcopyright} Georg Thieme Verlag KG Stuttgart · New York.",
year = "2017",
month = oct,
doi = "10.1055/s-0043-113042",
language = "English",
volume = "38",
pages = "857--863",
journal = "International journal of sports medicine",
issn = "0172-4622",
publisher = "Georg Thieme Verlag",
number = "11",

}

RIS

TY - JOUR

T1 - Serotonin Shapes the Migratory Potential of NK Cells

T2 - An in vitro Approach

AU - Zimmer, Philipp

AU - Bloch, Wilhelm

AU - Kieven, Markus

AU - Lövenich, Lukas

AU - Lehmann, Jonas

AU - Holthaus, Michelle

AU - Theurich, Sebastian

AU - Schenk, Alexander

N1 - © Georg Thieme Verlag KG Stuttgart · New York.

PY - 2017/10

Y1 - 2017/10

N2 - Increased serotonin (5-HT) levels have been shown to influence natural killer cell (NK cell) function. Acute exercise mobilizes and activates NK cells and further increases serum 5-HT concentrations in a dose-dependent manner. The aim of this study was to investigate the impact of different serum 5-HT concentrations on NK cell migratory potential and cytotoxicity. The human NK cell line KHYG-1 was assigned to 4 conditions, including 3 physiological concentrations of 5-HT (100, 130 or 170 µg/l 5-HT) and one control condition. NK cells were analyzed regarding cytotoxicity, migratory potential and expression of adhesion molecules. No treatment effect on NK cell cytotoxicity and expression of integrin subunits was detected. Migratory potential was increased in a dose dependent manner, indicating the highest protease activity in cells that were incubated with 170 µg/l 5-HT (170 µg/l vs. control, p<0.001, 170 µg/l vs. 100 µg/l, p<0.001; 170 µg/l vs. 130 µg/l, p=0.003; 130 µg/l vs. control, p<0.001, 130 µg/l vs. 100 µg/l, p<0.001). These results suggest that elevated 5-HT serum levels play a mediating role in NK cell function. As exercise has been shown to be involved in NK cell mobilization and redistribution, the influence of 5-HT should be investigated in ex vivo and in vivo experiments.

AB - Increased serotonin (5-HT) levels have been shown to influence natural killer cell (NK cell) function. Acute exercise mobilizes and activates NK cells and further increases serum 5-HT concentrations in a dose-dependent manner. The aim of this study was to investigate the impact of different serum 5-HT concentrations on NK cell migratory potential and cytotoxicity. The human NK cell line KHYG-1 was assigned to 4 conditions, including 3 physiological concentrations of 5-HT (100, 130 or 170 µg/l 5-HT) and one control condition. NK cells were analyzed regarding cytotoxicity, migratory potential and expression of adhesion molecules. No treatment effect on NK cell cytotoxicity and expression of integrin subunits was detected. Migratory potential was increased in a dose dependent manner, indicating the highest protease activity in cells that were incubated with 170 µg/l 5-HT (170 µg/l vs. control, p<0.001, 170 µg/l vs. 100 µg/l, p<0.001; 170 µg/l vs. 130 µg/l, p=0.003; 130 µg/l vs. control, p<0.001, 130 µg/l vs. 100 µg/l, p<0.001). These results suggest that elevated 5-HT serum levels play a mediating role in NK cell function. As exercise has been shown to be involved in NK cell mobilization and redistribution, the influence of 5-HT should be investigated in ex vivo and in vivo experiments.

KW - Cell Line

KW - Cell Movement

KW - Exercise

KW - Humans

KW - Integrins

KW - Killer Cells, Natural

KW - Serotonin

KW - Journal Article

U2 - 10.1055/s-0043-113042

DO - 10.1055/s-0043-113042

M3 - Journal articles

C2 - 28783845

VL - 38

SP - 857

EP - 863

JO - International journal of sports medicine

JF - International journal of sports medicine

SN - 0172-4622

IS - 11

ER -

ID: 3264094