Strength training alters MCT1-protein expression and exercise-induced translocation in erythrocytes of men with non-insulin-dependent type-2 diabetes.

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Strength training alters MCT1-protein expression and exercise-induced translocation in erythrocytes of men with non-insulin-dependent type-2 diabetes. / Opitz, David; Kreutz, Thorsten; Lenzen, Edward; Dillkofer, Benedict; Wahl, Patrick; Montiel Garcia, Georgina; Graf, Christine; Bloch, Wilhelm; Brixius, Klara.

in: Canadian journal of physiology and pharmacology, Jahrgang 92, Nr. 3, 03.2014, S. 259-262.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

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@article{4d492a3d93504842ba1d53b691f15581,
title = "Strength training alters MCT1-protein expression and exercise-induced translocation in erythrocytes of men with non-insulin-dependent type-2 diabetes.",
abstract = "We investigated the cellular distribution of lactate transporter (MCT1) and its chaperone CD147 (using immunohistochemistry and fluorescence-activated cell sorting) in the erythrocytes of men with non-insulin-dependent type-2 diabetes (NIDDM, n = 11, 61 ± 8 years of age) under acute exercise (ergometer cycling test, World Health Organisation scheme) performed before and after a 3-month strength training program. Cytosolic MCT1 distribution and membraneous CD147 density did not change after acute exercise (ergometer). After the 3-month strength training, MCT1-density was increased and the reaction of MCT1 (but not that of CD147) towards acute exercise (ergometer) was altered. MCT1 localisation was shifted from the centre to the cellular membrane. This resulted in a decrease in the immunohistochemically measured cytosolic MCT1-density. We conclude that strength training alters the acute exercise reaction of MCT1 but not that of CD147 in erythrocytes in patients with NIDDM. This reaction may contribute to long-term normalisation and stabilisation of the regulation of lactate plasma concentration in NIDDM.",
keywords = "Aged, Antigens, CD147, Diabetes Mellitus, Type 2, Erythrocytes, Humans, Insulin, Male, Middle Aged, Monocarboxylic Acid Transporters, Protein Transport, Resistance Training, Symporters",
author = "David Opitz and Thorsten Kreutz and Edward Lenzen and Benedict Dillkofer and Patrick Wahl and {Montiel Garcia}, Georgina and Christine Graf and Wilhelm Bloch and Klara Brixius",
year = "2014",
month = "3",
doi = "10.1139/cjpp-2012-0405",
language = "English",
volume = "92",
pages = "259--262",
journal = "Canadian journal of physiology and pharmacology",
issn = "0008-4212",
publisher = "National Research Council of Canada",
number = "3",

}

RIS

TY - JOUR

T1 - Strength training alters MCT1-protein expression and exercise-induced translocation in erythrocytes of men with non-insulin-dependent type-2 diabetes.

AU - Opitz, David

AU - Kreutz, Thorsten

AU - Lenzen, Edward

AU - Dillkofer, Benedict

AU - Wahl, Patrick

AU - Montiel Garcia, Georgina

AU - Graf, Christine

AU - Bloch, Wilhelm

AU - Brixius, Klara

PY - 2014/3

Y1 - 2014/3

N2 - We investigated the cellular distribution of lactate transporter (MCT1) and its chaperone CD147 (using immunohistochemistry and fluorescence-activated cell sorting) in the erythrocytes of men with non-insulin-dependent type-2 diabetes (NIDDM, n = 11, 61 ± 8 years of age) under acute exercise (ergometer cycling test, World Health Organisation scheme) performed before and after a 3-month strength training program. Cytosolic MCT1 distribution and membraneous CD147 density did not change after acute exercise (ergometer). After the 3-month strength training, MCT1-density was increased and the reaction of MCT1 (but not that of CD147) towards acute exercise (ergometer) was altered. MCT1 localisation was shifted from the centre to the cellular membrane. This resulted in a decrease in the immunohistochemically measured cytosolic MCT1-density. We conclude that strength training alters the acute exercise reaction of MCT1 but not that of CD147 in erythrocytes in patients with NIDDM. This reaction may contribute to long-term normalisation and stabilisation of the regulation of lactate plasma concentration in NIDDM.

AB - We investigated the cellular distribution of lactate transporter (MCT1) and its chaperone CD147 (using immunohistochemistry and fluorescence-activated cell sorting) in the erythrocytes of men with non-insulin-dependent type-2 diabetes (NIDDM, n = 11, 61 ± 8 years of age) under acute exercise (ergometer cycling test, World Health Organisation scheme) performed before and after a 3-month strength training program. Cytosolic MCT1 distribution and membraneous CD147 density did not change after acute exercise (ergometer). After the 3-month strength training, MCT1-density was increased and the reaction of MCT1 (but not that of CD147) towards acute exercise (ergometer) was altered. MCT1 localisation was shifted from the centre to the cellular membrane. This resulted in a decrease in the immunohistochemically measured cytosolic MCT1-density. We conclude that strength training alters the acute exercise reaction of MCT1 but not that of CD147 in erythrocytes in patients with NIDDM. This reaction may contribute to long-term normalisation and stabilisation of the regulation of lactate plasma concentration in NIDDM.

KW - Aged

KW - Antigens, CD147

KW - Diabetes Mellitus, Type 2

KW - Erythrocytes

KW - Humans

KW - Insulin

KW - Male

KW - Middle Aged

KW - Monocarboxylic Acid Transporters

KW - Protein Transport

KW - Resistance Training

KW - Symporters

U2 - 10.1139/cjpp-2012-0405

DO - 10.1139/cjpp-2012-0405

M3 - Journal articles

VL - 92

SP - 259

EP - 262

JO - Canadian journal of physiology and pharmacology

T2 - Canadian journal of physiology and pharmacology

JF - Canadian journal of physiology and pharmacology

SN - 0008-4212

IS - 3

ER -

ID: 64848