Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Standard

Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men. / Brinkmann, C; Chung, N; Schmidt, U; Kreutz, T; Lenzen, E; Schiffer, T; Geisler, S; Graf, C; Montiel Garcia, Georgina; Renner, R; Bloch, W; Brixius, K.

in: Scandinavian journal of medicine & science in sports, Jahrgang 22, Nr. 4, 01.08.2012, S. 462-70.

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschungBegutachtung

Harvard

APA

Vancouver

Bibtex

@article{8c1e6562a5d84083a254b04aea47b643,
title = "Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men",
abstract = "The present study analyzes the oxidative stress situation in the skeletal muscle of overweight/obese men suffering from non-insulin-dependent type 2 diabetes mellitus [T2DM, n=16, years=61±7, body mass index (BMI)=31±4 kg/m(2) ] and BMI-matched non-diabetic male control subjects (CON, n=7, years=53±6, BMI=30±4 kg/m(2) ). Furthermore, it investigates whether physical training can alter the skeletal muscle antioxidative capacity of T2DM patients at rest. Molecule content analyses (immunohistochemical stainings) of 8-iso-prostaglandin-F2α (8-Iso-PGF), superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), peroxiredoxin isoforms (PRDX 1-6) and heat-shock-protein-70 (HSP70) were performed in biopsies taken from the vastus lateralis muscle. Under basal conditions, 8-Iso-PGF was significantly decreased in T2DM patients (-35.7{\%}), whereas PRDX2 and PRDX6 were significantly increased relative to CON (+82.6{\%}; +82.3{\%}). Differences were neither observed in SOD2 nor in GPX1 or PRDX1, 3, 4, 5 density. Regular physical activity (moderate endurance or resistance training twice a week for 3 months) did not alter PRDX1, 2, 3, 4, 6 in the skeletal muscle of T2DM patients, but significantly increased SOD2 (+65.9{\%}), GPX1 (+62.4{\%}), PRDX5 (+37.5{\%}), and HSP70 (+48.5{\%}). Overweight/obese men with non-insulin-dependent T2DM exhibit up-regulated cytosolic peroxiredoxin contents relative to BMI-matched controls. Regular training further up-regulates cytosolic and mitochondrial antioxidative enzymes in T2DM patients and improves their cellular protection systems. This may contribute to a retardation of the disease's progression.",
author = "C Brinkmann and N Chung and U Schmidt and T Kreutz and E Lenzen and T Schiffer and S Geisler and C Graf and {Montiel Garcia}, Georgina and R Renner and W Bloch and K Brixius",
note = "{\circledC} 2011 John Wiley & Sons A/S.",
year = "2012",
month = "8",
day = "1",
doi = "10.1111/j.1600-0838.2010.01273.x",
language = "English",
volume = "22",
pages = "462--70",
journal = "Scandinavian journal of medicine & science in sports",
issn = "1600-0838",
publisher = "Blackwell Munksgaard",
number = "4",

}

RIS

TY - JOUR

T1 - Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men

AU - Brinkmann, C

AU - Chung, N

AU - Schmidt, U

AU - Kreutz, T

AU - Lenzen, E

AU - Schiffer, T

AU - Geisler, S

AU - Graf, C

AU - Montiel Garcia, Georgina

AU - Renner, R

AU - Bloch, W

AU - Brixius, K

N1 - © 2011 John Wiley & Sons A/S.

PY - 2012/8/1

Y1 - 2012/8/1

N2 - The present study analyzes the oxidative stress situation in the skeletal muscle of overweight/obese men suffering from non-insulin-dependent type 2 diabetes mellitus [T2DM, n=16, years=61±7, body mass index (BMI)=31±4 kg/m(2) ] and BMI-matched non-diabetic male control subjects (CON, n=7, years=53±6, BMI=30±4 kg/m(2) ). Furthermore, it investigates whether physical training can alter the skeletal muscle antioxidative capacity of T2DM patients at rest. Molecule content analyses (immunohistochemical stainings) of 8-iso-prostaglandin-F2α (8-Iso-PGF), superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), peroxiredoxin isoforms (PRDX 1-6) and heat-shock-protein-70 (HSP70) were performed in biopsies taken from the vastus lateralis muscle. Under basal conditions, 8-Iso-PGF was significantly decreased in T2DM patients (-35.7%), whereas PRDX2 and PRDX6 were significantly increased relative to CON (+82.6%; +82.3%). Differences were neither observed in SOD2 nor in GPX1 or PRDX1, 3, 4, 5 density. Regular physical activity (moderate endurance or resistance training twice a week for 3 months) did not alter PRDX1, 2, 3, 4, 6 in the skeletal muscle of T2DM patients, but significantly increased SOD2 (+65.9%), GPX1 (+62.4%), PRDX5 (+37.5%), and HSP70 (+48.5%). Overweight/obese men with non-insulin-dependent T2DM exhibit up-regulated cytosolic peroxiredoxin contents relative to BMI-matched controls. Regular training further up-regulates cytosolic and mitochondrial antioxidative enzymes in T2DM patients and improves their cellular protection systems. This may contribute to a retardation of the disease's progression.

AB - The present study analyzes the oxidative stress situation in the skeletal muscle of overweight/obese men suffering from non-insulin-dependent type 2 diabetes mellitus [T2DM, n=16, years=61±7, body mass index (BMI)=31±4 kg/m(2) ] and BMI-matched non-diabetic male control subjects (CON, n=7, years=53±6, BMI=30±4 kg/m(2) ). Furthermore, it investigates whether physical training can alter the skeletal muscle antioxidative capacity of T2DM patients at rest. Molecule content analyses (immunohistochemical stainings) of 8-iso-prostaglandin-F2α (8-Iso-PGF), superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), peroxiredoxin isoforms (PRDX 1-6) and heat-shock-protein-70 (HSP70) were performed in biopsies taken from the vastus lateralis muscle. Under basal conditions, 8-Iso-PGF was significantly decreased in T2DM patients (-35.7%), whereas PRDX2 and PRDX6 were significantly increased relative to CON (+82.6%; +82.3%). Differences were neither observed in SOD2 nor in GPX1 or PRDX1, 3, 4, 5 density. Regular physical activity (moderate endurance or resistance training twice a week for 3 months) did not alter PRDX1, 2, 3, 4, 6 in the skeletal muscle of T2DM patients, but significantly increased SOD2 (+65.9%), GPX1 (+62.4%), PRDX5 (+37.5%), and HSP70 (+48.5%). Overweight/obese men with non-insulin-dependent T2DM exhibit up-regulated cytosolic peroxiredoxin contents relative to BMI-matched controls. Regular training further up-regulates cytosolic and mitochondrial antioxidative enzymes in T2DM patients and improves their cellular protection systems. This may contribute to a retardation of the disease's progression.

U2 - 10.1111/j.1600-0838.2010.01273.x

DO - 10.1111/j.1600-0838.2010.01273.x

M3 - Journal articles

C2 - 21477162

VL - 22

SP - 462

EP - 470

JO - Scandinavian journal of medicine & science in sports

JF - Scandinavian journal of medicine & science in sports

SN - 1600-0838

SN - 0905-7188

IS - 4

ER -

ID: 166128