VEGF coordinates interaction of pericytes and endothelial cells during vasculogenesis and experimental angiogenesis

Publikationen: Beitrag in FachzeitschriftZeitschriftenaufsätzeForschung

AutorInnen

  • Martin Hagedorn
  • Maurice Balke
  • Annette Schmidt
  • Wilhelm Bloch
  • Haymo Kurz
  • Sophie Javerzat
  • Benoît Rousseau
  • Joerg Wilting
  • Andreas Bikfalvi

Forschungseinrichtungen

Details

Biological activities of vascular endothelial growth factor (VEGF) have been studied extensively in endothelial cells (ECs), but few data are available regarding its effects on pericytes. In murine embryoid body cultures, VEGF-induced expression of desmin and alpha-smooth muscle actin (alpha-SMA) in CD-31+ cells. The number of CD-31+/desmin+ vascular chords increased with VEGF treatment time and peaked during a differentiation window between 6 and 9 days after plating. In vivo, VEGF-induced elongation and migration of desmin-positive pericytes and coverage of angiogenic capillaries, as revealed by analysis of Sambucus nigra lectin-stained vascular beds of the chick chorioallantoic membrane. VEGF also caused significant decrease of intercapillary spaces, an indicator for intussusceptive vascular growth. These VEGF-mediated effects point at a more intricate interaction between ECs and pericytes cells than previously demonstrated and suggest that pericytes may be derived from EC progenitors in vitro and not only stabilize capillaries but also participate in vascular remodeling in vivo.

OriginalspracheEnglisch
ZeitschriftDevelopmental dynamics : an official publication of the American Association of Anatomists
Jahrgang230
Heft1
Seiten (von - bis)23-33
Seitenumfang11
ISSN1058-8388
DOIs
PublikationsstatusVeröffentlicht - 01.05.2004

ID: 71995

DOI

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