Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells

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Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells. / Schenk, Alexander; Pulverer, Walter; Koliamitra, Christine; Bauer, Claus Juergen; Ilic, Suzana; Heer, Rudolf; Schier, Robert; Schick, Volker; Böttiger, Bernd W; Gerhäuser, Clarissa; Bloch, Wilhelm; Zimmer, Philipp.

In: International journal of sports medicine, Vol. 40, No. 1, 01.2019, p. 62-70.

Research output: Contribution to journalJournal articlesResearchpeer-review

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Schenk, A, Pulverer, W, Koliamitra, C, Bauer, CJ, Ilic, S, Heer, R, Schier, R, Schick, V, Böttiger, BW, Gerhäuser, C, Bloch, W & Zimmer, P 2019, 'Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells', International journal of sports medicine, vol. 40, no. 1, pp. 62-70. https://doi.org/10.1055/a-0741-7001

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@article{63bded73f0294fefa92a3364e89b3776,
title = "Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells",
abstract = "Positive effects of exercise on cancer prevention and progression have been proposed to be mediated by stimulating natural killer (NK) cells. Because NK cell receptors are regulated by epigenetic modifications, we investigated whether acute aerobic exercise and training change promoter DNA methylation and gene expression of the activating KIR2DS4 and the inhibiting KIR3DL1 gene. Sixteen healthy women (50-60 years) performed a graded exercise test (GXT) and were randomized into either a passive control group or an intervention group performing a four-week endurance exercise intervention. Blood samples (pre-, post-GXT and post-training) were used for isolation of DNA/RNA of NK cells to assess DNA promoter methylation by targeted deep-amplicon sequencing and gene expression by qRT-PCR. Potential changes in NK cell subsets were determined by flow cytometry. Acute and chronic exercise did not provoke significant alterations of NK cell proportions. Promoter methylation decreased and gene expression increased for KIR2DS4 after acute exercise. A high gene expression correlated with a low methylation of CpGs that were altered by acute exercise. Chronic exercise resulted in a minor decrease of DNA methylation and did not alter gene expression. Acute exercise provokes epigenetic modifications, affecting the balance between the activating KIR2DS4 and the inhibiting KIR3DL1, with potential benefits on NK cell function.",
keywords = "DNA Methylation, Demethylation, Epigenesis, Genetic, Exercise, Exercise Test, Female, Gene Expression, Humans, Killer Cells, Natural, Middle Aged, Promoter Regions, Genetic, Receptors, KIR, Journal Article, Randomized Controlled Trial",
author = "Alexander Schenk and Walter Pulverer and Christine Koliamitra and Bauer, {Claus Juergen} and Suzana Ilic and Rudolf Heer and Robert Schier and Volker Schick and B{\"o}ttiger, {Bernd W} and Clarissa Gerh{\"a}user and Wilhelm Bloch and Philipp Zimmer",
note = "{\circledC} Georg Thieme Verlag KG Stuttgart · New York.",
year = "2019",
month = "1",
doi = "10.1055/a-0741-7001",
language = "English",
volume = "40",
pages = "62--70",
journal = "International journal of sports medicine",
issn = "0172-4622",
publisher = "Georg Thieme Verlag",
number = "1",

}

RIS

TY - JOUR

T1 - Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells

AU - Schenk, Alexander

AU - Pulverer, Walter

AU - Koliamitra, Christine

AU - Bauer, Claus Juergen

AU - Ilic, Suzana

AU - Heer, Rudolf

AU - Schier, Robert

AU - Schick, Volker

AU - Böttiger, Bernd W

AU - Gerhäuser, Clarissa

AU - Bloch, Wilhelm

AU - Zimmer, Philipp

N1 - © Georg Thieme Verlag KG Stuttgart · New York.

PY - 2019/1

Y1 - 2019/1

N2 - Positive effects of exercise on cancer prevention and progression have been proposed to be mediated by stimulating natural killer (NK) cells. Because NK cell receptors are regulated by epigenetic modifications, we investigated whether acute aerobic exercise and training change promoter DNA methylation and gene expression of the activating KIR2DS4 and the inhibiting KIR3DL1 gene. Sixteen healthy women (50-60 years) performed a graded exercise test (GXT) and were randomized into either a passive control group or an intervention group performing a four-week endurance exercise intervention. Blood samples (pre-, post-GXT and post-training) were used for isolation of DNA/RNA of NK cells to assess DNA promoter methylation by targeted deep-amplicon sequencing and gene expression by qRT-PCR. Potential changes in NK cell subsets were determined by flow cytometry. Acute and chronic exercise did not provoke significant alterations of NK cell proportions. Promoter methylation decreased and gene expression increased for KIR2DS4 after acute exercise. A high gene expression correlated with a low methylation of CpGs that were altered by acute exercise. Chronic exercise resulted in a minor decrease of DNA methylation and did not alter gene expression. Acute exercise provokes epigenetic modifications, affecting the balance between the activating KIR2DS4 and the inhibiting KIR3DL1, with potential benefits on NK cell function.

AB - Positive effects of exercise on cancer prevention and progression have been proposed to be mediated by stimulating natural killer (NK) cells. Because NK cell receptors are regulated by epigenetic modifications, we investigated whether acute aerobic exercise and training change promoter DNA methylation and gene expression of the activating KIR2DS4 and the inhibiting KIR3DL1 gene. Sixteen healthy women (50-60 years) performed a graded exercise test (GXT) and were randomized into either a passive control group or an intervention group performing a four-week endurance exercise intervention. Blood samples (pre-, post-GXT and post-training) were used for isolation of DNA/RNA of NK cells to assess DNA promoter methylation by targeted deep-amplicon sequencing and gene expression by qRT-PCR. Potential changes in NK cell subsets were determined by flow cytometry. Acute and chronic exercise did not provoke significant alterations of NK cell proportions. Promoter methylation decreased and gene expression increased for KIR2DS4 after acute exercise. A high gene expression correlated with a low methylation of CpGs that were altered by acute exercise. Chronic exercise resulted in a minor decrease of DNA methylation and did not alter gene expression. Acute exercise provokes epigenetic modifications, affecting the balance between the activating KIR2DS4 and the inhibiting KIR3DL1, with potential benefits on NK cell function.

KW - DNA Methylation

KW - Demethylation

KW - Epigenesis, Genetic

KW - Exercise

KW - Exercise Test

KW - Female

KW - Gene Expression

KW - Humans

KW - Killer Cells, Natural

KW - Middle Aged

KW - Promoter Regions, Genetic

KW - Receptors, KIR

KW - Journal Article

KW - Randomized Controlled Trial

U2 - 10.1055/a-0741-7001

DO - 10.1055/a-0741-7001

M3 - Journal articles

C2 - 30508863

VL - 40

SP - 62

EP - 70

JO - International journal of sports medicine

JF - International journal of sports medicine

SN - 0172-4622

IS - 1

ER -

ID: 3644516