The aim of this study was to investigate the relationship between plantar pressure pattern and wearing comfort of five different prefabricated insoles. Therefore wearing comfort was assessed by a questionnaire and the use of a visual analogue scale (VAS) and plantar pressure was measured by a Pedar-X system. For statistical analysis single factor ANOVA for repeated measurements, Bonferroni and Pearson correlation test were performed. Significant differences in comfort ratings and average maximum plantar pressure were found. A relationship between average plantar pressure and comfort could not be detected. Nevertheless prefabricated insoles are able to improve wearing comfort of running shoes. Additional parameters like insole hardness and/or flexibility might also influence wearing comfort and seems to modulate the plantar pressure pattern during running.